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Concerns that repeated anti\u2010helminthic treatment may lead to drug resistance render it important that vaccines are developed but will require increased understanding of the immune\u2010mediated cellular and antibody responses to helminth infection. IL\u20104 or antibody\u2010activated murine macrophages are known to immobilize parasitic nematode larvae, but few studies have addressed whether this is translatable to human macrophages. In the current study, we investigated the capacity of human macrophages to recognize and attack larval stages of\n                    <jats:italic>Ascaris suum<\/jats:italic>\n                    , a natural porcine parasite that is genetically similar to the human helminth\n                    <jats:italic>Ascaris lumbricoides<\/jats:italic>\n                    . Human macrophages were able to adhere to and trap\n                    <jats:italic>A\u00a0suum<\/jats:italic>\n                    larvae in the presence of either human or pig serum containing\n                    <jats:italic>Ascaris<\/jats:italic>\n                    \u2010specific antibodies and other factors. Gene expression analysis of serum\u2010activated macrophages revealed that CCL24, a potent eosinophil attractant, was the most upregulated gene following culture with\n                    <jats:italic>A\u00a0suum<\/jats:italic>\n                    larvae in vitro, and human eosinophils displayed even greater ability to adhere to, and trap,\n                    <jats:italic>A\u00a0suum<\/jats:italic>\n                    larvae. These data suggest that immune serum\u2013activated macrophages can recruit eosinophils to the site of infection, where they act in concert to immobilize tissue\u2010migrating\n                    <jats:italic>Ascaris<\/jats:italic>\n                    larvae.\n                  <\/jats:p>","DOI":"10.1111\/pim.12728","type":"journal-article","created":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T02:09:31Z","timestamp":1589249371000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Immune serum\u2013activated human macrophages coordinate with eosinophils to immobilize\n                    <i>Ascaris suum<\/i>\n                    larvae"],"prefix":"10.1111","volume":"42","author":[{"given":"Gillian","family":"Coakley","sequence":"first","affiliation":[{"name":"Department of Immunology and Pathology Central Clinical School Monash University Melbourne Victoria 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