{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:28:20Z","timestamp":1776904100676,"version":"3.51.2"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T00:00:00Z","timestamp":1593388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006449","name":"Fundaci\u00f3n Alberto J. Roemmers","doi-asserted-by":"publisher","award":["\u2010"],"award-info":[{"award-number":["\u2010"]}],"id":[{"id":"10.13039\/501100006449","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The aim of this study was to evaluate characteristics of B cells in human tegumentary leismaniasis (TL) analysing cutaneous leishmaniasis (CL), most prevalent form and mucosal leishmaniasis (ML), aggressive form characterized by the destruction of the oral\u2010nasal\u2010pharyngeal cavities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>By flow cytometry analysis, we found decreased percentages of non\u2013class\u2010switched memory B cells in TL with the degree of the loss related to clinical severity. Using commercial ELISA, we reported high levels of B\u2010cell activating factor (BAFF) and IgG preferentially in aggressive CL and markedly in ML together with decreased BAFF receptors in the latter. We also found lower levels of BAFF after clinical recovery suggesting a relation between BAFF and disease activity.<\/jats:p><jats:p>Mucosal leishmaniasis history of therapeutic failure presented high levels of BAFF accompanied by detectable concentrations of IFN\u2010\u03b3 and IL\u20106 (assayed by commercial ELISA and cytometric bead arrays respectively), cytokines involved in exaggerated inflammatory responses and tissue damage in TL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We demonstrate B\u2010cell disturbances in TL with the degree of the alterations related to clinical severity. We suggest a relation between excess of BAFF and disease activity and point towards a possible implication of BAFF in the inflammatory phenomenon of ML.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pim.12759","type":"journal-article","created":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T22:08:40Z","timestamp":1590617320000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["B\u2010cell profile, B\u2010cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5589-1443","authenticated-orcid":false,"given":"Cecilia","family":"Parodi","sequence":"first","affiliation":[{"name":"Instituto de Patolog\u00eda Experimental\u2010CONICET Universidad Nacional de Salta Salta Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra","family":"Barrio","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Microbiolog\u00eda Facultad de Ciencias de la Salud Universidad Nacional de Salta Salta Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda F.","family":"Garc\u00eda Bustos","sequence":"additional","affiliation":[{"name":"Instituto de Patolog\u00eda Experimental\u2010CONICET Universidad Nacional de Salta Salta Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana G.","family":"Gonz\u00e1lez Prieto","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Microbiolog\u00eda Facultad de Ciencias de la Salud Universidad Nacional de Salta Salta Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Pimentel","sequence":"additional","affiliation":[{"name":"Instituto de Patolog\u00eda Experimental\u2010CONICET Universidad Nacional de Salta Salta Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noel","family":"Badano","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunolog\u00eda Instituto de Medicina Experimental\u2010CONICET Academia Nacional de Medicina Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda C.","family":"Albareda","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Parasitolog\u00eda Dr. M. Fatala Chaben Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melisa E.","family":"Castro Eiro","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Parasitolog\u00eda Dr. M. Fatala Chaben Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana A.","family":"Laucella","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Parasitolog\u00eda Dr. M. Fatala Chaben Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda M.","family":"de Elizalde de Bracco","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunolog\u00eda Instituto de Medicina Experimental\u2010CONICET Academia Nacional de Medicina Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2017.10.001"},{"issue":"5","key":"e_1_2_10_3_1","first-page":"2009","article-title":"Promotion of a functional B cell germinal center response after leishmania species co\u2010infection is associated with lesion resolution","volume":"180","author":"Gibson\u2010Corley KN","year":"2012","journal-title":"AJPA"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2007.08.010"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1521-6616(03)00092-5"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3810"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2572"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000082106"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2006.04.004"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-08-301887"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302960"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.07218"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01338"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.14-0260"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8019.2009.01272.x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1586\/eri.10.19"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.77.636"},{"issue":"1","key":"e_1_2_10_18_1","first-page":"30","article-title":"Identificaci\u00f3n por PS\u2010PCR de especies de Leishmania y su correlaci\u00f3n con caracter\u00edsticas cl\u00ednicas, epidemiol\u00f3gicas y terap\u00e9uticas en Salta, Argentina","volume":"1","author":"Barrio A","year":"2009","journal-title":"Rev Argentina Salud P\u00fablica"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-016-0455-0"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(95)90144-2"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2011.11.011"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.11.008"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-10-362"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keq029"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1086\/590215"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.21.120601.141152"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0807841105"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/0961203315608254"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2006.065656"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir438"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200535537"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2013.12.016"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.12.6734-6740.2002"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2017.11.001"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/0961203309106492"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpim.12759","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12759","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pim.12759","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12759","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T05:26:45Z","timestamp":1693891605000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12759"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,29]]},"references-count":34,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1111\/pim.12759"],"URL":"https:\/\/doi.org\/10.1111\/pim.12759","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,29]]},"assertion":[{"value":"2019-10-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12759"}}