{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T03:59:49Z","timestamp":1793332789883,"version":"4.5.0"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Giardia lamblia<\/jats:italic>\n                      is a protozoan parasite that causes giardiasis, one of the most common worldwide gastrointestinal diseases. For rational development of a\n                      <jats:italic>Giardia<\/jats:italic>\n                      vaccine, increasing our understanding of the host\u2010\n                      <jats:italic>Giardia<\/jats:italic>\n                      interaction is crucial. In this study, we analysed the immunogenicity and antigenicity of two\n                      <jats:italic>G lamblia<\/jats:italic>\n                      strain variants [GS and GS\u20105G8 (+)], which express different levels of the variant\u2010specific surface protein (VSP) 5G8 and also analysed the intestinal histological changes associated with\n                      <jats:italic>Giardia<\/jats:italic>\n                      infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      We evaluated the antibody responses induced by\n                      <jats:italic>G lamblia<\/jats:italic>\n                      strains in infected, reinfected and immunized C3H\/HeJ mice using ELISA, flow cytometry, Western blotting and histological analysis. Our results showed that\n                      <jats:italic>G lamblia<\/jats:italic>\n                      GS\u20105G8 (+) was more immunogenic and antigenic than the GS strain. The antibody response against the GS\u20105G8 (+) strain primarily recognized 5G8 protein. Serum antibody from infected and reinfected mice exhibited specific agglutination of trophozoites in vitro. GS\u20105G8 (+)\u2010infected mice showed higher CD19\n                      <jats:sup>+<\/jats:sup>\n                      infiltrating cell levels compared to GS\u2010infected animals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>G lamblia<\/jats:italic>\n                      strains with different expression levels of an immunogenic antigen (VSP 5G8) induce differential antibody responses. A better understanding of the immunogenic proteins of\n                      <jats:italic>G lamblia<\/jats:italic>\n                      will contribute to the rational development of an effective vaccine against this parasitic disease.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/pim.12767","type":"journal-article","created":{"date-parts":[[2020,6,28]],"date-time":"2020-06-28T11:45:34Z","timestamp":1593344734000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Differential antibody responses to\n                    <i>Giardia lamblia<\/i>\n                    strain variants expressing dissimilar levels of an immunogenic protein"],"prefix":"10.1111","volume":"42","author":[{"given":"Thania","family":"Garzon","sequence":"first","affiliation":[{"name":"Department of Chemistry\u2010Biology University of Sonora Hermosillo 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