{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T02:34:24Z","timestamp":1772505264464,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T00:00:00Z","timestamp":1596758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>We evaluate whether the serum and aqueous humour (AH) level of IgG anti\u2010Hsp70.1 antibodies improved the biological diagnosis of ocular toxoplasmosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      In this prospective cross\u2010sectional and multicentre study, serum and AH were collected at the time of active uveitis. Anti\u2010Hsp70.1\u2010antibody levels were determined by ELISA. Patients with confirmed (Group A1, n\u00a0=\u00a021) or suspected ocular toxoplasmosis (group A2, n\u00a0=\u00a030) were enrolled, as well as a control group of patients with cataract (group B, n\u00a0=\u00a042). Serum IgG anti\u2010Hsp70.1 antibody levels were not significantly different within the group of uveitis patients (A1, n\u00a0=\u00a021 vs A2, n\u00a0=\u00a030,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.8) and were significantly associated with the affected retinal zone (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.006) and with the size of the retinal lesion (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.03). Serum anti\u2010Hsp70.1 antibody level was positive in 10 out of the 18 patients of group A2. Significant anti\u2010Hsp\u201070.1 antibody level in AH was reported in only three patients (3 eyes) with confirmed ocular toxoplasmosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>While the level of IgG anti\u2010Hsp\u201070.1 antibody in AH did not improve the laboratory diagnosis of ocular toxoplasmosis, its level in serum was of major significance for retinal damage diagnosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/pim.12771","type":"journal-article","created":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T10:16:19Z","timestamp":1593512179000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Prospective study of serum and aqueous humour anti\u2010Hsp70.1 IgG antibody levels in ocular toxoplasmosis"],"prefix":"10.1111","volume":"42","author":[{"given":"Christophe","family":"Chiquet","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Grenoble Alpes University Hospital Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernab\u00e9","family":"Chumpitazi","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Mycology CHU de Grenoble\u2010Alpes and Institute for Advanced Biosciences INSERM U1209 CNRS UMR5309 Grenoble Alpes University Hospital Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Vilgrain","sequence":"additional","affiliation":[{"name":"CNRS, INSERM, CEA Institute of Interdisciplinary Research of Grenoble (IRIG) Laboratory of Biology of Cancer and Infection Univ. Grenoble Alpes Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Lesoin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Grenoble Alpes University Hospital Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helene","family":"Fricker\u2010Hidalgo","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Mycology CHU de Grenoble\u2010Alpes and Institute for Advanced Biosciences INSERM U1209 CNRS UMR5309 Grenoble Alpes University Hospital Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Pierre","family":"Brenier\u2010Pinchart","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Mycology CHU de Grenoble\u2010Alpes and Institute for Advanced Biosciences INSERM U1209 CNRS UMR5309 Grenoble Alpes University Hospital Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Vasseneix","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Valence Hospital Valence France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Savy","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Chambery Hospital Chambery France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelly","family":"Campolmi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Saint Etienne University Hospital Saint Etienne France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Gain","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Saint Etienne University Hospital Saint Etienne France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Flori","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Mycology Saint\u2010Etienne University Hospital Saint Etienne France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florent","family":"Aptel","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Grenoble Alpes University Hospital Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herv\u00e9","family":"Pelloux","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Mycology CHU de Grenoble\u2010Alpes and Institute for Advanced Biosciences INSERM U1209 CNRS UMR5309 Grenoble Alpes University Hospital Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8245-4767","authenticated-orcid":false,"given":"Laurence","family":"Bouillet","sequence":"additional","affiliation":[{"name":"CNRS, INSERM, CEA Institute of Interdisciplinary Research of Grenoble (IRIG) Laboratory of Biology of Cancer and Infection Univ. 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