{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:37:20Z","timestamp":1762299440976,"version":"3.41.2"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T00:00:00Z","timestamp":1596758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Identification of a 29\u00a0kDa heat stress protein in filarial parasite <jats:italic>Setaria cervi<\/jats:italic> and evaluation of its diagnostic potential against lymphatic filariasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>The Heat shock proteins (HSPs) were induced in filarial parasite <jats:italic>S\u00a0cervi<\/jats:italic> by incubated at 42\u00b0C for 2\u00a0hours. The 10% SDS\u2010PAGE of cytosolic extract showed several over\u2010expressed bands. The MALDI\u2010LC\/MS analysis of 29\u00a0kDa band showed 100% similarity with <jats:italic>Bm<\/jats:italic>14\u20103\u20103 like protein 2. Multiple sequence alignment of <jats:italic>Bm<\/jats:italic>14\u20103\u20103 like protein 2 sequence with <jats:italic>W\u00a0bancrofti, Caenorhabditis elegans<\/jats:italic>; <jats:italic>Loa loa<\/jats:italic> and <jats:italic>Homo sapiens<\/jats:italic> showed 100%, 86%, 83% and 78%, sequence similarity respectively. The antigenic efficacy of <jats:italic>Sc<\/jats:italic>14\u20103\u20103 protein was evaluated with different filarial sera using ELISA which showed cross\u2010reactivity in order to Endemic Normal (EN)\u00a0&lt;\u00a0Microfilaraemic (MF)\u00a0&lt;\u00a0Chronic(CH) with IgG1 and EN\u00a0&lt;\u00a0CH\u00a0&lt;\u00a0MF in IgG4 ELISA. IgG1\u2010 and IgG4\u2010specific immunoblotting with CH and MF sera further explicated its specific antigenic cross\u2010reactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A 29\u00a0kDa heat shock protein of <jats:italic>S\u00a0cervi<\/jats:italic> was identified as 14\u20103\u20103 protein having 100% homology to human filarial parasite <jats:italic>B\u00a0malayi<\/jats:italic>. It showed strong reactivity with IgG1 and IgG4 subclass antibodies of <jats:italic>W\u00a0bancrofti<\/jats:italic>\u2010infected human sera suggesting that 14\u20103\u20103 protein could be used as a vaccine\/ diagnostic marker.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pim.12777","type":"journal-article","created":{"date-parts":[[2020,7,18]],"date-time":"2020-07-18T08:09:06Z","timestamp":1595059746000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of a HSP14\u20103\u20103 in <i>Setaria cervi<\/i> and its cross\u2010reactivity with <i>W\u00a0bancrofti<\/i>\u2010infected human sera"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4248-0981","authenticated-orcid":false,"given":"Faiyaz","family":"Ahmad","sequence":"first","affiliation":[{"name":"Department of Biochemistry Institute of Science Banaras Hindu University Varanasi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ranjeet","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Science Banaras Hindu University Varanasi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarika","family":"Gupta","sequence":"additional","affiliation":[{"name":"National institute of Immunology New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8687-2007","authenticated-orcid":false,"given":"Sushma","family":"Rathaur","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Science Banaras Hindu University Varanasi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,7]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"WHO.Global programme to eliminate lymphatic filariasis. progress report;2017."},{"key":"e_1_2_10_3_1","unstructured":"WHO.Alternative mass drug administration regimens to eliminate lymphatic filariasis. 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