{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:32:45Z","timestamp":1753878765924,"version":"3.41.2"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T00:00:00Z","timestamp":1604966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000341","name":"Arthritis Research UK","doi-asserted-by":"publisher","award":["21133"],"award-info":[{"award-number":["21133"]}],"id":[{"id":"10.13039\/501100000341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/M029662\/1","BB\/M029727\/1"],"award-info":[{"award-number":["BB\/M029662\/1","BB\/M029727\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>ES\u201062 is a well\u2010studied anti\u2010inflammatory molecule secreted by L4\u2010adult stage <jats:italic>Acanthocheilonema viteae<\/jats:italic>. We maintain the life cycle of <jats:italic>A\u00a0viteae<\/jats:italic> using <jats:italic>Meriones libycus<\/jats:italic> as the definitive host. Here, we investigated whether the full life cycle could be maintained, and functional ES\u201062 produced, in a related jird species\u2014<jats:italic>Meriones shawi<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>Adult worms were produced in comparable numbers in the two species, but very few microfilariae (MF) were observed in the <jats:italic>M\u00a0shawi<\/jats:italic> bloodstream. <jats:italic>M\u00a0shawi<\/jats:italic> ES\u201062 produced ex vivo was functional and protective in a mouse model of arthritis. Myeloid\u2010derived cells from na\u00efve and infected jirds of both species were compared with respect to ROS production and osteoclast generation, and some differences between the two species in both the absence and presence of infection were observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The life cycle of <jats:italic>A\u00a0viteae<\/jats:italic> cannot be successfully completed in <jats:italic>M\u00a0shawi<\/jats:italic> jirds but L3 stage worms develop to adulthood and produce functional ES\u201062. Preliminary investigation into jird immune responses suggests that infection can differentially modulate myeloid responses in the two species. However, species\u2010specific reagents are required to understand the complex interplay between <jats:italic>A\u00a0viteae<\/jats:italic> and its host and to explain the lack of circulating MF in infected <jats:italic>M\u00a0shawi<\/jats:italic> jirds.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pim.12803","type":"journal-article","created":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T17:45:40Z","timestamp":1603388740000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of <i>Acanthocheilonema viteae<\/i> in <i>Meriones shawi<\/i>: Absence of microfilariae and production of active ES\u201062"],"prefix":"10.1111","volume":"43","author":[{"given":"Felicity E.","family":"Lumb","sequence":"first","affiliation":[{"name":"Strathclyde Institute of Pharmacy and Biomedical Sciences University of Strathclyde Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Doonan","sequence":"additional","affiliation":[{"name":"Strathclyde Institute of Pharmacy and Biomedical Sciences University of Strathclyde Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marlene","family":"Corbet","sequence":"additional","affiliation":[{"name":"Institute of Infection, Immunity and Inflammation University of Glasgow Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel A.","family":"Pineda","sequence":"additional","affiliation":[{"name":"Institute of Infection, Immunity and Inflammation University of Glasgow Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret","family":"M. Harnett","sequence":"additional","affiliation":[{"name":"Institute of Infection, Immunity and Inflammation University of Glasgow Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9545-9401","authenticated-orcid":false,"given":"William","family":"Harnett","sequence":"additional","affiliation":[{"name":"Strathclyde Institute of Pharmacy and Biomedical Sciences University of Strathclyde Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2017.05.007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000058686"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002001920"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2014.03.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.4.2127"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.12.001"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008391"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00534937"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2307\/3275034"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01016"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.1997.d01-129.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1967.0079"},{"key":"e_1_2_9_15_1","first-page":"27","article-title":"Phosphorylcholine mimics the effects of ES\u201062 on macrophages and dendritic cells","volume":"29","author":"Goodridge HS","year":"2017","journal-title":"Parasit Immunol"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00010737"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003206"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1976.25.199"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12803","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pim.12803","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12803","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T06:11:33Z","timestamp":1693635093000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,10]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["10.1111\/pim.12803"],"URL":"https:\/\/doi.org\/10.1111\/pim.12803","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"type":"print","value":"0141-9838"},{"type":"electronic","value":"1365-3024"}],"subject":[],"published":{"date-parts":[[2020,11,10]]},"assertion":[{"value":"2020-08-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12803"}}