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EVs were purified of sera from four groups (5 A\/Sn mice\/group). EV\u2010IM: Mice immunized with\n                    <jats:italic>T\u00a0gondii<\/jats:italic>\n                    \u2010released EVs; ACT: mice in acute infection; CHR: mice in chronic infection; and NI: normal mice. EVs were purified by ultracentrifugation. Concentration of serum\u2010derived EVs from NI group was smaller than EV\u2010IM, ACT and CHR groups. Most of the EVs from ACT and CHR groups were microvesicles, and they were bigger than the NI group. The same results were shown by Transmission Electron Microscopy. The presence of exosomes was shown in immunoblotting by tetraspanin (CD63 and CD9) evidence. Splenocytes of EV\u2010IM, CHR and NI groups were stimulated with T. gondii derived EVs. EV\u2010IM and CHR groups up\u2010expressed IFN\u2010\u03b3; TNF\u2010\u03b1 and IL\u201017, when compared with the NI group. IL\u201010 was up\u2010expressed only in the EV\u2010IM group. EV\u2010IM, ACT and CHR groups expressed more miR\u2010155\u20105p, miR\u201029c\u20103p and miR\u2010125b\u20105p than the NI group. Host\u2010\n                    <jats:italic>T\u00a0gondii<\/jats:italic>\n                    interaction can occur, also, via EVs. miRNAs participate in the modulation of cellular immune response against\n                    <jats:italic>T\u00a0gondii<\/jats:italic>\n                    . These data give subsidies to propose the differentiation between infect or noninfect hosts by concentration of EVs.\n                  <\/jats:p>","DOI":"10.1111\/pim.12869","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T10:29:40Z","timestamp":1621852180000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Characterization of murine extracellular vesicles and\n                    <i>Toxoplasma gondii<\/i>\n                    infection"],"prefix":"10.1111","volume":"43","author":[{"given":"Marta Marques","family":"Maia","sequence":"first","affiliation":[{"name":"Centro de Parasitologia e Micologia Instituto Adolfo Lutz Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allecineia Bispo","family":"da Cruz","sequence":"additional","affiliation":[{"name":"Centro de Parasitologia e Micologia Instituto Adolfo Lutz Sao Paulo 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