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The objective of the current study was to investigate the immunotherapeutic activities of the hydroalcoholic extract of\n                      <jats:italic>Glycyrrhiza glabra<\/jats:italic>\n                      (HEG) and glycyrrhizic acid (GA) in the treatment of Leishmania major (\n                      <jats:italic>L. major<\/jats:italic>\n                      )\u2010infected BALB\/c mice. In this study, the effect of HEG and GA was checked\n                      <jats:italic>in vitro<\/jats:italic>\n                      on growth of\n                      <jats:italic>L. major<\/jats:italic>\n                      promastigote and amastigote using MTT assay and microscopic counting, respectively. For\n                      <jats:italic>in vivo<\/jats:italic>\n                      experiment, the lesion induced by\n                      <jats:italic>L. major<\/jats:italic>\n                      on BALB\/c mice were treated intraperitoneally with HEG, GA, meglumine antimoniate or phosphate buffer saline (negative control) for one month. Then, the lesion development and the parasite burden of the lymph node was assessed, the cytokine response (IFN\u2010\u03b3 and IL\u20104) to\n                      <jats:italic>Leishmania<\/jats:italic>\n                      antigens was evaluated using ELISA method. The results showed that HEG and GA significantly inhibited the growth of\n                      <jats:italic>L. major<\/jats:italic>\n                      promastigotes and amastigotes, the lesion development, parasite burden in the lymph nodes, level of IFN\u2010\u03b3 and the ratio of IFN\u2010\u03b3\/IL\u20104 in HEG, GA and meglumine antimoniate\u2010treated mice were significantly higher compared with the negative control group, there was no difference between the HEG, GA and meglumine antimoniate group. It is concluded that hydroalcoholic extract of\n                      <jats:italic>G. glabra<\/jats:italic>\n                      and glycyrrhizic acid showed therapeutic and immunomodulatory effects on\n                      <jats:italic>L. major<\/jats:italic>\n                      \u2010infected BALB\/c mice\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/pim.12879","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T12:40:49Z","timestamp":1632487249000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Immunotherapeutic effects of\n                    <i>Glycyrrhiza glabra<\/i>\n                    and Glycyrrhizic Acid on\n                    <i>Leishmania major<\/i>\n                    \u2010infection BALB\/C mice"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8060-7213","authenticated-orcid":false,"given":"Shima","family":"Sheikhi","sequence":"first","affiliation":[{"name":"Department of Immunology Faculty of Medicine Shahed University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3165-9337","authenticated-orcid":false,"given":"Ali","family":"Khamesipour","sequence":"additional","affiliation":[{"name":"Center for Research and Training in Skin Diseases and Leprosy Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2599-8340","authenticated-orcid":false,"given":"Tayebeh","family":"Radjabian","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Basic Sciences Shahed University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1049-7095","authenticated-orcid":false,"given":"Tooba","family":"Ghazanfari","sequence":"additional","affiliation":[{"name":"Immunoregulation Research Center Shahed University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0140-4122","authenticated-orcid":false,"given":"Akram","family":"Miramin Mohammadi","sequence":"additional","affiliation":[{"name":"Center for Research and Training in Skin Diseases and Leprosy Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035671"},{"key":"e_1_2_10_3_1","unstructured":"OrganizationWHReport of a meeting of the WHO Expert Committee on the Control of Leishmaniases Geneva Switzerland. 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