{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T04:45:22Z","timestamp":1758861922629,"version":"3.41.2"},"reference-count":55,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T00:00:00Z","timestamp":1633910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aims of this work were to evaluate the protective role of the 250\u2010kDa polypeptide band of <jats:italic>Naegleria fowleri<\/jats:italic>. We designed an immunization strategy in Balb\/c mice which were inoculated by i.n. route with an electrocuted 250\u2010kDa polypeptide band of <jats:italic>N<\/jats:italic>.\u00a0<jats:italic>fowleri<\/jats:italic>. We observed that the 250\u2010kDa band induced 80% of protection, whereas the coadministration with Cholera Toxin induced 100% of protection. Moreover, high levels of IgA\u2010 and IgG\u2010specific antibodies were detected by ELISA assay. We also analysed migration molecules (\u03b14\u03b21 and LFA\u20101) on T and B lymphocytes in nose\u2010associated lymphoid tissue (NALT), cervical lymph nodes (CN) and nasal passages (NP) by flow cytometry. We observed that the percentage of B cells (B220\/\u03b14\u03b21) and T cells (CD4\/\u03b14\u03b21) in NP were higher in all immunized groups compared with the other compartments analysed. Finally, we detected by immunohistochemistry ICAM\u20101 and V\u2010CAM\u20101 in the nasal cavity. The immunization with the 250\u2010kDa polypeptide band, protect mice against <jats:italic>N<\/jats:italic>.\u00a0<jats:italic>fowleri<\/jats:italic>\u00a0challenge and modifies migration molecules and their ligands.<\/jats:p>","DOI":"10.1111\/pim.12882","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T20:50:19Z","timestamp":1632775819000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A 250\u2010kDa glycoprotein of <i>Naegleria fowleri<\/i> induces protection and modifies the expression of \u03b14\u03b21 and LFA\u20101 on T and B lymphocytes in mouse meningitis model"],"prefix":"10.1111","volume":"43","author":[{"given":"Diego A.","family":"Castillo\u2010Ram\u00edrez","sequence":"first","affiliation":[{"name":"Instituto Polit\u00e9cnico Nacional Salvador D\u00edaz Mir\u00f3n esq. Plan de San Luis S\/N Miguel Hidalgo, Casco de Santo Tomas Escuela Superior de Medicina Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Maricela","family":"Carrasco\u2010Y\u00e9pez","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Ambiental Grupo CyMA, UIICSE, FES Iztacala, UNAM Estado de M\u00e9xico Tlalnepantla de Baz M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itzel Berenice","family":"Rodr\u00edguez\u2010Mera","sequence":"additional","affiliation":[{"name":"Instituto Polit\u00e9cnico Nacional Salvador D\u00edaz Mir\u00f3n esq. Plan de San Luis S\/N Miguel Hidalgo, Casco de Santo Tomas Escuela Superior de Medicina Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldo Arturo","family":"Res\u00e9ndiz\u2010Albor","sequence":"additional","affiliation":[{"name":"Instituto Polit\u00e9cnico Nacional Salvador D\u00edaz Mir\u00f3n esq. Plan de San Luis S\/N Miguel Hidalgo, Casco de Santo Tomas Escuela Superior de Medicina Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9rica","family":"Rosales\u2010Cruz","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Hematopatolog\u00eda Departamento de Morfolog\u00eda Escuela Nacional de Ciencias Biol\u00f3gicas M\u00e9xico City M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1936-2664","authenticated-orcid":false,"given":"Sa\u00fal","family":"Rojas\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto Polit\u00e9cnico Nacional Salvador D\u00edaz Mir\u00f3n esq. Plan de San Luis S\/N Miguel Hidalgo, Casco de Santo Tomas Escuela Superior de Medicina Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.583077"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11908-002-0037-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874279301810010047"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2224"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00521-6"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-015-0164-2"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ddtec.2020.11.003"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3012"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.04.090"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2535"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01229-14"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/31514"},{"key":"e_1_2_9_14_1","first-page":"117","volume-title":"Molecular Recognition of HIV Glycans by Antibodies","author":"Kong L","year":"2013"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9060460"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3549-2"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12508"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1303238"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-8932(03)76837-7"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00952"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.8.4368-4375.2004"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-004-1177-6"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1994.1023"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.032"},{"issue":"2","key":"e_1_2_9_25_1","first-page":"57","article-title":"Proteinases in Naegleria Fowleri (strain NF3), a pathogenic amoeba: a preliminary study","volume":"21","author":"Mat AN","year":"2004","journal-title":"Trop Biomed"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-1760-y"},{"key":"e_1_2_9_27_1","first-page":"275","volume-title":"Inmunolog\u00eda. Celular y Molecular","author":"Abbas AK","year":"2015"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.3.559-562.1987"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2014.04.002"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2007.00999.x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/019459989210600221"},{"issue":"1","key":"e_1_2_9_32_1","first-page":"3","article-title":"A histological study of bowman's glands in human olfactory mucosa","volume":"60","author":"Jafari F","year":"2010","journal-title":"Pak Armed Forces Med J"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00302"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-415847-4.00040-9"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90162-0"},{"issue":"3","key":"e_1_2_9_36_1","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.4049\/jimmunol.163.3.1382","article-title":"Nasal\u2010associated lymphoid tissue: phenotypic and functional evidence for the primary role of peripheral node addressin in naive lymphocyte adhesion to high endothelial venules in a mucosal site","volume":"163","author":"Csencsits KL","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI44262"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2014.46"},{"issue":"167","key":"e_1_2_9_39_1","first-page":"2824","article-title":"The affinity of integrin \u03b14\u03b21 governs lymphocyte migration","volume":"2001","author":"Rose D","year":"1950","journal-title":"J Immunol"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.3.501"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00242"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902407"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01494-13"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.9.1467"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2010.01222.x"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1159\/000489405"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.11.040193.004003"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.6.1553"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(94)90119-8"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/7.4.393"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1177\/1945892419836819"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.171.4.1369"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1159\/000226236"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115403"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V79.1.206.206"},{"issue":"3","key":"e_1_2_9_56_1","first-page":"313","article-title":"Immunolocalization of endothelial and leukocyte adhesion molecules in human rheumatoid and osteoarthritic synovial tissues","volume":"64","author":"Koch AE","year":"1991","journal-title":"Lab Invest"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12882","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pim.12882","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12882","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T06:54:13Z","timestamp":1693205653000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12882"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,11]]},"references-count":55,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1111\/pim.12882"],"URL":"https:\/\/doi.org\/10.1111\/pim.12882","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"type":"print","value":"0141-9838"},{"type":"electronic","value":"1365-3024"}],"subject":[],"published":{"date-parts":[[2021,10,11]]},"assertion":[{"value":"2021-02-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12882"}}