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T helper\u20109 (Th9) cells secrete interleukin (IL)\u20109, a signature cytokine associated with tumour immunology, allergy and autoimmunity. Nonetheless, the understanding of modulatory functions of BLAODA on Th9 and other immune cells is limited. In this study, the BLAODA gene was cloned, and the recombinant (r) protein of BLAODA (rHcBLAODA) was expressed and immunoblotting was performed. The results revealed that HcBLAODA gene was successfully cloned and rHcBLAODA protein was expressed. The localization of rHcBLAODA was confirmed on the surface of gut sections from adult <jats:italic>H. contortus<\/jats:italic>. The rHcBLAODA protein capability to react precisely with anti\u2010<jats:italic>H. contortus<\/jats:italic> antibodies were confirmed by immunoblotting and immunofluorescence assay (IFA). Further functional analysis showed that interaction of rHcBLAODA with host cells significantly enhanced Th9 cells generation, IL\u20109 expression, nitric oxide production and cell apoptosis while suppressing the cells proliferation and cells migration depending on the concentration. Overall, these findings suggest that rHcBLAODA protein could modulate the host immune response by inducing Th9 cells to secrete IL\u20109 cytokine <jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/pim.12895","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T19:06:03Z","timestamp":1634843163000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Characteristics of Biotin lipoyl attachment and 2\u2010oxoacid dehydrogenase acyltransferase of the parasitic nematode <i>Haemonchus contortus<\/i> and its modulatory functions on goat PBMCs <i>in vitro<\/i>"],"prefix":"10.1111","volume":"43","author":[{"given":"Kalibixiati","family":"Aimulajiang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia Xinjiang Medical University Urumqi Xinjiang China"},{"name":"MOE Joint 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