{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:54:46Z","timestamp":1772160886515,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T00:00:00Z","timestamp":1637107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017261","name":"Universidad Nacional Autonoma de Honduras","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In some central\u2010American countries,\n                    <jats:italic>Leishmania<\/jats:italic>\n                    (\n                    <jats:italic>L<\/jats:italic>\n                    .)\n                    <jats:italic>infantum chagasi<\/jats:italic>\n                    infection can cause non\u2010ulcerated or atypical cutaneous leishmaniasis (NUCL) in addition to the classic clinical form, visceral leishmaniasis (VL). Little is known about the host\u2010parasite relationship that can contribute to the determination of one or another clinical form. The present study had the objective to evaluate the humoral and cellular immunity in the sera of individuals affected by NUCL to improve the comprehension of this atypical host\u2010parasite interaction. Based on clinical and laboratory diagnosis, serum of 80 individuals was collected to evaluate the cytokines and immunoglobulins profile of NUCL (n\u00a0=\u00a047), VL patients (n\u00a0=\u00a05), and negative controls (n\u00a0=\u00a028). Cytokines were detected using Cytokine Bead Array (CBA) Human Th1\/Th2\/Th17\u00a0kit according to the manufacturer's instructions; class (IgG and IgM), and subclass of (IgG1 and IgG2) immunoglobulins was evaluated by ELISA using specific antigens. The concentration of TNF\u2010\u03b1, IFN\u2010\u03b3, IL\u20102 and IL\u20104 cytokines in NUCL, VL and control was present below the detection threshold of CBA kit. IL\u20106, IL\u201010 and IL\u201017A cytokines was lower in NUCL compared to LV patients. Regarding to immunoglobulins, NUCL patients produced 4.0 times more IgG than the control, while VL patients produced 6.6 times more; and IgM level was 1.6 times higher in NUCL and 2.6 times in VL patients compared to the control. Concerning the immunoglobulins subclass, only VL patients showed positive reaction for IgG1, and IgG2 did not show positive reaction among the groups. The results showed a weak cellular and humoral systemic immune response in NUCL patients.\n                  <\/jats:p>","DOI":"10.1111\/pim.12896","type":"journal-article","created":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T12:28:36Z","timestamp":1636374516000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of systemic immunity in atypical cutaneous leishmaniasis caused by\n                    <i>Leishmania (L.) infantum chagasi<\/i>"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1080-2440","authenticated-orcid":false,"given":"M\u00e1rcia Dalastra","family":"Laurenti","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Patologia de Mol\u00e9stias Infecciosas (LIM\u201050) Departamento de Patologia Faculdade de Medicina Universidade de S\u00e3o Paulo S\u00e3o Paulo SP Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1387-7488","authenticated-orcid":false,"given":"Wilfredo","family":"Sosa\u2010Ochoa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia de Mol\u00e9stias Infecciosas (LIM\u201050) Departamento de Patologia Faculdade de Medicina Universidade de S\u00e3o Paulo S\u00e3o Paulo SP Brasil"},{"name":"Instituto de Investigaciones en Microbiolog\u00eda Universidad Nacional Aut\u00f3noma de Honduras Tegucigalpa Honduras"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8560-7298","authenticated-orcid":false,"given":"Gabriela Venicia","family":"Araujo Flores","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia de Mol\u00e9stias Infecciosas (LIM\u201050) Departamento de Patologia Faculdade de Medicina Universidade de S\u00e3o Paulo S\u00e3o Paulo SP Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6566-4869","authenticated-orcid":false,"given":"Carmen Maria","family":"Sandoval Pacheco","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia de Mol\u00e9stias Infecciosas (LIM\u201050) Departamento de Patologia Faculdade de Medicina Universidade de S\u00e3o Paulo S\u00e3o Paulo SP Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1431-6829","authenticated-orcid":false,"given":"Thaise Yumie","family":"Tomokane","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia de Mol\u00e9stias Infecciosas (LIM\u201050) Departamento de Patologia Faculdade de Medicina Universidade de S\u00e3o Paulo S\u00e3o Paulo SP Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luanda Mara da Silva","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Dermatologia e Imunodefici\u00eancias (LIM\u201056) Departamento de Dermatologia, Faculdade de Medicina e Instituto de Medicina Tropical de S\u00e3o Paulo Universidade de S\u00e3o Paulo S\u00e3o Paulo SP Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2622-792X","authenticated-orcid":false,"given":"Concepci\u00f3n","family":"Z\u00faniga","sequence":"additional","affiliation":[{"name":"Departamento de Vigilancia de la Salud Hospital Escuela Tegucigalpa Honduras"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0412-6060","authenticated-orcid":false,"given":"Fernando Tobias","family":"Silveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia Instituto Evandro Chagas (Minist\u00e9rio da Sa\u00fade) Bel\u00e9m PA Brasil"},{"name":"N\u00facleo de Medicina Tropical Universidade Federal do Par\u00e1 Bel\u00e9m PA Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1587-3085","authenticated-orcid":false,"given":"Carlos Eduardo Pereira","family":"Corbett","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia de Mol\u00e9stias Infecciosas (LIM\u201050) Departamento de Patologia Faculdade de Medicina Universidade de S\u00e3o Paulo S\u00e3o Paulo SP Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.61.380"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90734-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(82)90295-4"},{"key":"e_1_2_8_5_1","first-page":"180","volume-title":"Manual de manejo de enfermedades parasitarias prioritarias en 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