{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:55:05Z","timestamp":1772160905060,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,5,14]],"date-time":"2022-05-14T00:00:00Z","timestamp":1652486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004685","name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004685","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Entamoeba histolytica<\/jats:italic>\n                    causes amoebic liver abscess (ALA) in humans. The injury of target cells by\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    includes processes controlled by the ubiquitin Ehub. Previously, we found immunodominance of Ehub glycan moieties using immunized rabbits. In this work, we analysed dominance of antibodies to the glycoprotein Ehub in the sera from 52 patients with ALA. Controls were sera from 20 healthy people living in endemic areas with a high seroprevalence of antibodies to amoebas, and 20 patients with alcoholic hepatitis (AH) to rule out the cross\u2010reaction of Ehub with autoantibodies induced by liver damage. Antigens were trophozoite extract, glycoprotein Ehub and the recombinant protein\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    recombinant ubiquitin (rEhub). The sera from healthy volunteers and patients with AH do not have antibodies to glycoprotein Ehub. Surprisingly, only the antibodies from patients with ALA recognized the glycoprotein Ehub, and some sera gave a faint reaction with the recombinant protein, especially because evolutionarily, the ubiquitin is conserved between species. This is the first report demonstrating that antibodies to ubiquitin Ehub are induced exclusively in patients with invasive amoebiasis, and the antibody response is mainly to the glycoprotein, indicating glycans are immunodominant. Inhibitors of the Ehub glycans could be potential treatment for amoebiasis by selectively damaging trophozoites.\n                  <\/jats:p>","DOI":"10.1111\/pim.12919","type":"journal-article","created":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T05:04:41Z","timestamp":1648875881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Ubiquitin of\n                    <i>Entamoeba histolytica<\/i>\n                    induces antibody response in patients with invasive amoebiasis"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6402-8235","authenticated-orcid":false,"given":"Mar\u00eda S.","family":"Flores","sequence":"first","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Facultad de Ciencias Biol\u00f3gicas Instituto de Biotecnolog\u00eda  San Nicol\u00e1s de los Garza Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4806-8640","authenticated-orcid":false,"given":"Eva","family":"Tamez","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n Facultad de Medicina  Monterrey Mexico"},{"name":"Veterinary Medicine Teaching Hospital University of California, Davis  Davis California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9088-7957","authenticated-orcid":false,"given":"Roberto","family":"Rangel","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Facultad de Ciencias Biol\u00f3gicas Instituto de Biotecnolog\u00eda  San Nicol\u00e1s de los Garza Mexico"},{"name":"Department of Head and Neck Surgery University of Texas MD Anderson Cancer Center  Houston Texas 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