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Through the separation and identification of the sporozoite membrane protein of <jats:italic>Cryptosporidium parvum<\/jats:italic> (<jats:italic>C. parvum<\/jats:italic>), 20 related proteins were obtained. Among them, a calmodulin\u2010like protein (CML) has a similar functional domain\u2010exchange factor hand (EF\u2010hand) motif as calmodulin proteins (CaMs), so it may play a similarly important role in the invasion process. A 663\u2009bp full gene encoding the <jats:italic>C. parvum<\/jats:italic> calmodulin\u2010like protein (CpCML) was inserted in pET28a vector and expressed in <jats:italic>Escherichia coli<\/jats:italic>. An immunofluorescence assay showed that CpCML was mainly located on the surface of the sporozoites. Three\u2010week\u2010old female BALB\/c mice were used for modelling the immunoreactions and immunoprotection of recombinant CpCML (rCpCML) against artificial <jats:italic>Cryptosporidium tyzzeri<\/jats:italic> infections. The results indicated a significantly increased in anti\u2010CpCML antibody response, which was induced by the immunized recombinant protein. Compared to rP23 (recombinant P23), GST6P\u20101 (expressed by pGEX\u20106P\u20101 transfected <jats:italic>E. coli<\/jats:italic>), GST4T\u20101 (expressed by pGEX\u20104T\u20101 transfected <jats:italic>E. coli<\/jats:italic>), glutathione (GSH), adjuvant and blank control groups, rCpCML\u2010immunized mice produced specific spleen cell proliferation in addition to different production levels of IL\u20102, IFN\u2010\u03b3, TNF\u2010\u03b1, IL\u20104 and IL\u20105. Additionally, immunization with rCpCML led to 34.08% reduction of oocyst shedding in <jats:italic>C. tyzzeri<\/jats:italic> infected mice faeces which was similar to rP23. These results suggest that CpCML may be developed as a potential vaccine candidate antigen against cryptosporidiosis.<\/jats:p>","DOI":"10.1111\/pim.12937","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T07:31:27Z","timestamp":1654155087000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Isolation and identification of sporozoite membrane protein of <i>Cryptosporidium parvum<\/i> and evaluation of calmodulin\u2010like protein immune protection"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1891-2086","authenticated-orcid":false,"given":"Yan","family":"Huang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture Shanghai Veterinary Research Institute, Chinese Academy 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