{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:54:46Z","timestamp":1772160886387,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T00:00:00Z","timestamp":1659484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Leishmaniasis includes several clinical forms. While routine diagnosis of cutaneous leishmaniasis (CL) is by microscopy, an antibody response to CL has been reported in several recent studies. This study evaluated anti\u2010leishmanial immunoglobulin G (IgG) antibody responses as a biomarker of active leishmaniasis and a measure of exposure to\n                    <jats:italic>Leishmania<\/jats:italic>\n                    . Sera from 50 untreated CL patients, 140 patients under treatment and 280 healthy individuals residing in endemic regions collected as part of an epidemiological survey, was analysed with an enzyme\u2010linked immunosorbent assay established in\u2010house using receiver operator characteristic curve at optimized cut\u2010off value. The assay showed high performance as a diagnostic tool in identifying exposure in endemic individuals (sensitivity: 98%, specificity: 90.3%). All patients showed lower antibody levels over time since onset of lesion\/s. Antibody levels were higher (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0\u02c2\u00a0.01) and persisted for a longer period in untreated patients. In patients under treatment, the level of anti\u2010IgG antibodies was negatively correlated with the total duration the patient had been on treatment. The anti\u2010leishmanial IgG response in\n                    <jats:italic>Leishmania donovani<\/jats:italic>\n                    \u2010induced CL is transient and is unlikely to confer protective immunity. Optimized serological assays may be useful in endemic settings for diagnosis and monitoring the treatment response in CL.\n                  <\/jats:p>","DOI":"10.1111\/pim.12940","type":"journal-article","created":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T01:29:19Z","timestamp":1657848559000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp>ELISA<\/scp>\n                    \u2010based evaluation of antibody response to\n                    <i>Leishmania<\/i>\n                    in a region endemic for cutaneous leishmaniasis"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5773-6033","authenticated-orcid":false,"given":"Sachee Bhanu","family":"Piyasiri","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine University of Colombo  Colombo Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5654-1189","authenticated-orcid":false,"given":"Thisum Nilakshi","family":"Samaranayake","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine University of Colombo  Colombo Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2288-1294","authenticated-orcid":false,"given":"Hermali","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine University of Colombo  Colombo Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4630-9399","authenticated-orcid":false,"given":"Nuwani Harshamali","family":"Manamperi","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine University of Kelaniya  Ragama Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3985-1817","authenticated-orcid":false,"given":"Nadira Darshani","family":"Karunaweera","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine University of Colombo  Colombo Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,3]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"WHO.Leishmaniasis.2022. 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