{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:54:46Z","timestamp":1772160886986,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T00:00:00Z","timestamp":1673049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["SB\/YS\/LS\u2010234\/2013"],"award-info":[{"award-number":["SB\/YS\/LS\u2010234\/2013"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"Abstract<\/jats:title>\n \n Visceral leishmaniasis (VL) is a neglected disease with a broad spectrum of clinical manifestations and involvement of visceral organs. Organ\u2010specific immune response against the\n Leishmania donovani<\/jats:italic>\n (\n Ld<\/jats:italic>\n ) complex is not yet understood due to the unavailability of an appropriate experimental model. In reference to our recent work on comparing the hamster model with VL patients, it is now possible to understand immune profiling in different visceral organs. This may offer an answer to varying parasite loads in different visceral organs in the same host. Herein, we analysed a panel of immune markers (Th\u20102\/Th\u20101) in visceral organs of\n Ld\u2010<\/jats:italic>\n infected hamsters and quantified parasitic load in the same tissues using qPCR assay. In spleen, liver, bone marrow and lymph node (mesenteric) from\n Ld\u2010<\/jats:italic>\n infected hamsters, the parasite burden was quantified along with mRNA expression of a panel of Th\u20102 and Th\u20101 type immune markers, namely IL\u201010, IL\u20104, Arginase\u2010I, GATA\u20103, SOCS\u20103, IL\u201012, IFN\u2010\u03b3, iNOS, T\u2010bet and SOCS\u20105. A clear dichotomy was absent between Th\u20102 and Th\u20101 type immune markers and the major players of this immune response were IFN\u2010\u03b3, IL\u201010, T\u2010bet, GATA\u20103, SOCS\u20105 and SOCS\u20103.\n <\/jats:p>","DOI":"10.1111\/pim.12964","type":"journal-article","created":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T07:10:51Z","timestamp":1672038651000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Organ\u2010specific immune profiling of\n Leishmania donovani<\/i>\n \u2010infected hamsters"],"prefix":"10.1111","volume":"45","author":[{"given":"Sheetal","family":"Saini","sequence":"first","affiliation":[{"name":"Department of Biotechnology Motilal Nehru National Institute of Technology (MNNIT) Allahabad Praygraj India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bharat","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Motilal Nehru National Institute of Technology (MNNIT) Allahabad Praygraj India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anuradha","family":"Dube","sequence":"additional","affiliation":[{"name":"Division of Parasitology CSIR\u2010Central Drug Research Institute (CSIR\u2010CDRI) Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amogh Anant","family":"Sahasrabuddhe","sequence":"additional","affiliation":[{"name":"Molecular & Structural Biology Division CSIR\u2010Central Drug Research Institute (CSIR\u2010CDRI) Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6564-6342","authenticated-orcid":false,"given":"Ambak Kumar","family":"Rai","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Motilal Nehru National Institute of Technology (MNNIT) Allahabad Praygraj India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,7]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"WHO. 2022. Updated January 8 2022. June 1 2022.www.who.int\/en\/news-room\/fact-sheets\/detail\/leishmaniasis"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12768"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11686-019-00127-8"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12783"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00153"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1412-x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2020.04.024"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2022.03.002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prostaglandins.2019.106390"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00406"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040940"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M508608200"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.2.4.425"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.202477099"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00156"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900412"},{"issue":"2","key":"e_1_2_7_18_1","first-page":"169","article-title":"Yellow fever virus infection in Syrian golden hamsters: relationship between cytokine expression and pathologic changes","volume":"1","author":"Li G","year":"2008","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000582"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12964","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pim.12964","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12964","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T21:58:19Z","timestamp":1692309499000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12964"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,7]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1111\/pim.12964"],"URL":"https:\/\/doi.org\/10.1111\/pim.12964","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/PIM.12964\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/PIM.12964\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/PIM.12964\/v1\/review1","asserted-by":"object"}]},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,7]]},"assertion":[{"value":"2022-09-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12964"}}