{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:54:47Z","timestamp":1772160887644,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T00:00:00Z","timestamp":1681776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A sensitive and specific diagnostic kit is crucial for the detection of human lymphatic filariasis at the early stage of infection as the existing diagnostic tools are inefficient and expensive. In the present study, we have cloned and expressed\n                    <jats:italic>Brugia malayi<\/jats:italic>\n                    HSP70 (BmHSP70) protein and characterized it as a potential antigen for diagnosis of the asymptomatic microfilariae stage of\n                    <jats:italic>Wuchereria. bancrofti<\/jats:italic>\n                    infection using ELISA, western blot, and bioinformatics tools. The antigenic efficacy of BmHSP70 was also compared with ScHSP70. The BmHSP70 and ScHSP70 peptide showed highly antigenic in nature and they showed immunogenic cross\u2010reactivity endemic normal (EN)\u2009&lt;\u2009chronic (CH)\u2009&lt;\u2009microfilaraemic (MF) in IgG, IgG1, and IgG4 ELISA. IgG4\u2010specific immunoblotting of BmHSP70 with MF sera further explicated its stage\u2010specific antigenic cross\u2010reactivity. These antigens (ScHSP70 and BmHSP70) showed a positive immunogenic correlation with the number of MF in blood samples. Thus, proposing BmHSP70 as a potential immunodiagnostic antigen against lymphatic filariasis. A triplet of GGMP tetrapeptide specific to the filarial HSP70 was also identified which was absent in human HSP70. In terms of sensitivity and specificity of antigens, these results suggest that recombinant BmHSP70 is a good antigen and could be used to diagnose early\u2010stage of microfilariae infection.\n                  <\/jats:p>","DOI":"10.1111\/pim.12978","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T01:12:52Z","timestamp":1681866772000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Human immune response against filarial\n                    <scp>HSP70<\/scp>\n                    and its role in the diagnosis of lymphatic filariasis"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4248-0981","authenticated-orcid":false,"given":"Faiyaz","family":"Ahmad","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Institute of Science Banaras Hindu University  Varanasi 221005 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Liebau","sequence":"additional","affiliation":[{"name":"Institute for Zoophysiology University of M\u00fcnster  Schlossplatz 8 M\u00fcnster D\u201048143 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8687-2007","authenticated-orcid":false,"given":"Sushma","family":"Rathaur","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Science Banaras Hindu University  Varanasi 221005 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,4,18]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"WHO.Ending the neglect to attain the sustainable development goals\u2014a road map for neglected tropical diseases 2021\u20132030.2020."},{"issue":"43","key":"e_1_2_10_3_1","first-page":"509","article-title":"Global programme to eliminate lymphatic filariasis: 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