{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T19:36:08Z","timestamp":1792438568030,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T00:00:00Z","timestamp":1716768000000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100024018","name":"Programa de Desarrollo de las Ciencias B\u00e1sicas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100024018","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006049","name":"Comisi\u00f3n Sectorial de Investigaci\u00f3n Cient\u00edfica","doi-asserted-by":"publisher","award":["C153\u2010348"],"award-info":[{"award-number":["C153\u2010348"]}],"id":[{"id":"10.13039\/501100006049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cystic echinococcosis is caused by the tissue\u2010dwelling larva (hydatid) of\n                    <jats:italic>Echinococcus granulosus sensu lato<\/jats:italic>\n                    . A salient feature is that this larva is protected by the acellular laminated layer (LL). As the parasite grows, the LL sheds abundant particles that can accumulate in the parasite's vicinity. The potential of LL particles to induce inflammation in vivo has not been specifically analysed. It is not known how each of its two major components, namely highly glycosylated mucins and calcium inositol hexakisphosphate (Ins\n                    <jats:italic>P<\/jats:italic>\n                    <jats:sub>6<\/jats:sub>\n                    ) deposits, impacts inflammation induced by the LL as a whole. In this work, we show that LL particles injected intraperitoneally cause infiltration of eosinophils, neutrophils and monocytes\/macrophages as well as the disappearance of resident (large peritoneal) macrophages. Strikingly, the absence of calcium Ins\n                    <jats:italic>P<\/jats:italic>\n                    <jats:sub>6<\/jats:sub>\n                    enhanced the recruitment of all the inflammatory cell types analysed. In contrast, oxidation of the mucin carbohydrates caused decreased recruitment of neutrophils. The carbohydrate\u2010oxidised particles caused cell influx nonetheless, which may be explained by possible receptor\u2010independent effects of LL particles on innate immune cells, as suggested by previous works from our group. In summary, LL particles can induce acute inflammatory cell recruitment partly dependent on its mucin glycans, and this recruitment is attenuated by the calcium Ins\n                    <jats:italic>P<\/jats:italic>\n                    <jats:sub>6<\/jats:sub>\n                    component.\n                  <\/jats:p>","DOI":"10.1111\/pim.13040","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T10:14:22Z","timestamp":1716804862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Acute Inflammatory Potential of Particles From the\n                    <i>Echinococcus granulosus<\/i>\n                    Laminated Layer Is Moderated by Its Calcium Inositol Hexakisphosphate Component"],"prefix":"10.1111","volume":"46","author":[{"given":"Leticia","family":"Grezzi","sequence":"first","affiliation":[{"name":"\u00c1rea Inmunolog\u00eda, Departamento de Biociencias (Facultad de Qu\u00edmica) Instituto de Higiene, Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Laboratorio de Inmunolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica (Facultad de Ciencias) Instituto de Higiene, Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Independent Researcher"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5375-6766","authenticated-orcid":false,"given":"\u00c1lvaro","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"\u00c1rea Inmunolog\u00eda, Departamento de Biociencias (Facultad de Qu\u00edmica) Instituto de Higiene, Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Laboratorio de Inmunolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica (Facultad de Ciencias) Instituto de Higiene, Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Casaravilla","sequence":"additional","affiliation":[{"name":"\u00c1rea Inmunolog\u00eda, Departamento de Biociencias (Facultad de Qu\u00edmica) Instituto de Higiene, Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Laboratorio de Inmunolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica (Facultad de Ciencias) Instituto de Higiene, Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.07.001"},{"key":"e_1_2_9_3_1","first-page":"121","article-title":"Immunology of Cystic Echinococcosis (Hydatid Disease)","volume":"124","author":"D\u00edaz \u00c1.","year":"2017","journal-title":"British Medical Bulletin"},{"key":"e_1_2_9_4_1","first-page":"87","article-title":"Immunology of a Unique Biological Structure: The Echinococcus Laminated Layer","volume":"14","author":"D\u00edaz A.","year":"2023","journal-title":"Protein & Cell"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2011.01.008"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2010.12.012"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2015.03.019"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2010.07.008"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2006.05328.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20262"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","unstructured":"F.Irigo\u00edn F.Ferreira C.Fern\u00e1ndez R. B.Sim andA.D\u00edaz \u201cMyo\u2010Inositol Hexakisphosphate Is a Major Component of an Extracellular Structure in the Parasitic CestodeEchinococcus granulosus \u201d8(2002).","DOI":"10.1042\/0264-6021:3620297"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008921"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","DOI":"10.1128\/iai.00031-23","article-title":"Mucins Shed From the Laminated Layer in Cystic Echinococcosis Are Captured by Kupffer Cells via the Lectin Receptor Clec4F","volume":"91","author":"Barrios A. 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