{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:55:00Z","timestamp":1772160900574,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T00:00:00Z","timestamp":1719187200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Pathogen recognition is an essential component to achieve the desired outcome of host protection. Nod\u2010like receptor pyrin containing domain 3 (NLRP3) is a cytoplasmic pattern recognition receptor (PRR) with a wide array of agonists, such as PAMPs, DAMPs, ATP, bacterial product and viral products. Stimulation of the NLRP3 inflammasome results in proteolytic activation of IL\u20101\u03b2 and IL\u201018, cell pyroptosis and classically, the induction of proinflammatory responses. St. Croix (STC) sheep have resistance traits exhibiting the appropriate T\u2010helper type 2 immune response ensuing protection during helminth parasitic infection whereas parasite\u2010susceptible Suffolk (SUF) sheep have an impaired response resulting in parasite establishment and adverse symptoms. The objective of these experiments was to determine if NLRP3 protein in H. contortus\u2010infected SUF sheep was defective using the classical activation pathway of NLRP3 inflammasome. Peripheral blood mononuclear cells (PBMCs) derived from H. contortus\u2010infected STC and SUF sheep were isolated from whole blood and treated (MCC950 treatment for 2\u2009h followed by LPS treatment for 3\u2009h, 1400\u2009W treatment for 2\u2009h followed by LPS treatment for 3\u2009h, LPS treatment for 3\u2009h or culture media for 3\u2009h). qPCR analysis of LPS\u2010stimulated PBMC revealed an upregulation in inflammatory associated genes IL\u20101\u03b2, TLR4, TNF\u03b1 and NF\u03baB (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001) in STC PBMC and downregulation in IFN\u03b3, IL\u20106 and iNOS for SUF PBMC. Pharmacological inhibition of iNOS in SUF PBMC resulted in an upregulation in the expression of IFN\u03b3. These preliminary data begin to discover a relationship between NLRP3 activation and TLR4 signalling in PBMC of STC and SUF sheep.\n                  <\/jats:p>","DOI":"10.1111\/pim.13054","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T10:10:29Z","timestamp":1719223829000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Variants of\n                    <scp>NLRP3<\/scp>\n                    Protein in\n                    <i>Haemonchus contortus<\/i>\n                    Infected Sheep: Impact on Immune Cell Responsiveness to\n                    <scp>LPS<\/scp>\n                    In Vitro"],"prefix":"10.1111","volume":"46","author":[{"given":"Denzel","family":"Middleton","sequence":"first","affiliation":[{"name":"Division of Animal and Nutritional Sciences West Virginia University  Morgantown West Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly","family":"Hanlon","sequence":"additional","affiliation":[{"name":"Division of Animal and Nutritional Sciences West Virginia University  Morgantown West Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott\u00a0P.","family":"Greiner","sequence":"additional","affiliation":[{"name":"Department of Animal and Poultry Sciences Virginia Tech  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2624-8835","authenticated-orcid":false,"given":"Scott\u00a0A.","family":"Bowdridge","sequence":"additional","affiliation":[{"name":"Division of Animal and Nutritional Sciences West Virginia University  Morgantown West Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02379"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1812779115"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2005.02.004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2004.01976.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(00)00653-6"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1413-8"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00548\u201017"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1636"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900640"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2010.01211.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/162158"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201055447\u20106"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(00)00189-6"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006022"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12697"},{"issue":"1","key":"e_1_2_9_17_1","first-page":"102","article-title":"Serum Interleukin\u20104 (IL\u20104) Production is Associated With Lower Fecal Egg Count in Parasite\u2010Resistant Sheep","volume":"211","author":"Jacobs J. R.","year":"2016","journal-title":"Veterinary Parasitology"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(97)00094-0"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/sky288"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000142249.08135.e9"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901363"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.13054","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T14:29:51Z","timestamp":1719412191000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.13054"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":21,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1111\/pim.13054"],"URL":"https:\/\/doi.org\/10.1111\/pim.13054","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/PIM.13054\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/PIM.13054\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/PIM.13054\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/PIM.13054\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/PIM.13054\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/PIM.13054\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/PIM.13054\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/PIM.13054\/v3\/response1","asserted-by":"object"}]},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"2023-11-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13054"}}