{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:55:00Z","timestamp":1772160900514,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["25088"],"award-info":[{"award-number":["25088"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We aimed to develop an indirect enzyme\u2010linked immunosorbent assay (ELISA) to evaluate the presence of specific IgG against\n                    <jats:italic>Toxocara canis<\/jats:italic>\n                    and\n                    <jats:italic>Toxocara cati<\/jats:italic>\n                    somatic antigens on the serum of patients with toxocariasis. The sensitivity, specificity, positive and negative predictive values for indirect\u2010ELISA were calculated by receiver operating characteristic curve (ROC) analysis and Youden's\n                    <jats:italic>J<\/jats:italic>\n                    using Likelihood ratio. All statistics were analysed and graphs are plotted using GraphPad Prism version 8.4.3 (Graph Pad Software, La Jolla, CA, USA), with 95% confidence interval (CI). The sensitivity, specificity, positive and negative predictive values for\n                    <jats:italic>T. canis<\/jats:italic>\n                    were 100%, 82%, 79% and 100%, respectively. The mentioned variables for\n                    <jats:italic>T. cati<\/jats:italic>\n                    were 97%, 82%, 78% and 98%, respectively. Five immune reactive bands of 38, 40, 72, 100 and 250\u2009kDa were common in both species.\n                    <jats:italic>Toxocara<\/jats:italic>\n                    crude antigens were highly immunogenic in human sera. Immunoreactive bands against\n                    <jats:italic>T. canis<\/jats:italic>\n                    compared to\n                    <jats:italic>T. cati<\/jats:italic>\n                    somatic antigen were about two times more. Unlike\n                    <jats:italic>Toxocara<\/jats:italic>\n                    excretory\u2010secretory antigen, that was homologue in two species, somatic antigens of\n                    <jats:italic>T. canis and T. cati<\/jats:italic>\n                    showed different immunoreactive bands in our western blot.\n                  <\/jats:p>","DOI":"10.1111\/pim.13055","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T07:40:03Z","timestamp":1720510803000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Somatic Antigens of Adult\n                    <i>Toxocara<\/i>\n                    helminthes for Detection of Human Toxocariasis"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7799-5072","authenticated-orcid":false,"given":"Zahra","family":"Navi","sequence":"first","affiliation":[{"name":"Department of Parasitology and Mycology School of Public Health, Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1842-2952","authenticated-orcid":false,"given":"Reza","family":"Falak","sequence":"additional","affiliation":[{"name":"Immunology Research Center, Institute of Immunology and Infection Diseases Iran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4164-9514","authenticated-orcid":false,"given":"Mehdi","family":"Mohebali","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Public Health, Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1030-4652","authenticated-orcid":false,"given":"Mohammad\u00a0Bagher","family":"Molairad","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Public Health, Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zabihollah","family":"Zarei","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Public Health, Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mojgan","family":"Aryaeipour","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Public Health, Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2443-7218","authenticated-orcid":false,"given":"Abbas","family":"Rahimi\u00a0Foroushani","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics School of Public Health, Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2265-7460","authenticated-orcid":false,"given":"Mohammad","family":"Zibaei","sequence":"additional","affiliation":[{"name":"Department of Parasitology School of Medicine, Alborz University of Medical Sciences  Karaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1048-2512","authenticated-orcid":false,"given":"Mohammad\u00a0Bagher","family":"Rokni","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Public Health, Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(17)30331\u20106"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2017.10.009"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2018.07.087"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.2.265\u2010272.2003"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jpids\/pit066"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmm.IJMM_18_259"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010020\u201004113\u20102"},{"key":"e_1_2_11_9_1","volume-title":"StatPearls [Internet]","author":"Winders W. 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