{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:42:14Z","timestamp":1753879334718,"version":"3.41.2"},"reference-count":58,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/18252\u20102"],"award-info":[{"award-number":["2019\/18252\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Interleukin 27 (IL\u201027) is a cytokine that regulates susceptibility to <jats:italic>Leishmania infantum<\/jats:italic> infection in humans and experimental models. This cytokine has not yet been described in canine leishmaniasis (CanL). Therefore, we investigated whether IL\u201027 has a regulatory role in CanL. The EBI3 and p28 subunits of IL\u201027 were measured in splenic leukocytes culture supernatant from dogs with CanL and compared to control dogs. We also correlated EBI3 and p28 levels with IL\u201021, anti\u2010<jats:italic>L. infantum<\/jats:italic> antibodies and parasite loads. We performed functional assays followed by IL\u201027 blockade and measured parasite loads, production of cytokines in splenic leukocytes culture supernatant, and the expression of PD\u20101, CTLA\u20104, phospho\u2010Stat\u20101\/3, T\u2010bet, GATA3 and nitric oxide production (NO). Both IL\u201027 subunits increased in the supernatant of dogs with CanL compared to control dogs. EBI3 and p28 levels showed a moderate positive correlation with IL\u201021 (<jats:italic>r<\/jats:italic>\u2009=\u20090.67, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001 and <jats:italic>r<\/jats:italic>\u2009=\u20090.45, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.012, respectively), and the EBI3 subunit was positively associated with anti\u2010<jats:italic>L. infantum<\/jats:italic> IgG antibodies (<jats:italic>r<\/jats:italic>\u2009=\u20090.38, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.040) and parasite load (<jats:italic>r<\/jats:italic>\u2009=\u20090.47, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.009). IL\u201027 and IL\u201021 participate of immune responses in CanL. IL\u201027 may be associated with the failure of immunity to control parasite replication via upregulation of the expression of PD\u20101, CTLA\u20104, T\u2010bet and NO in splenic leukocytes from dogs with CanL. These findings suggest that the pathways regulated by IL\u201027 are involved in CanL pathogenesis in the host, and may be targets for new therapies.<\/jats:p>","DOI":"10.1111\/pim.13063","type":"journal-article","created":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T12:30:16Z","timestamp":1727958616000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Interleukin\u201027 Regulates Adaptative Immune Responses Associated With Control of Parasite Replication in Canine Leishmaniasis"],"prefix":"10.1111","volume":"46","author":[{"given":"Marilene Oliveira","family":"dos Santos","sequence":"first","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sidnei Ferro","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela Torres","family":"Rebech","sequence":"additional","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e9ssica Henrique","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaqueline Poleto","family":"Bragato","sequence":"additional","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matheus Fujimura","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas Takeshi Siqueira","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavia","family":"de Rezende Eug\u00eanio","sequence":"additional","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo S\u00e9rgio Patto","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9ria Mar\u00e7al Felix","family":"de Lima","sequence":"additional","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,3]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"World Health Organization (WHO) Leishmaniasis(2023). accessed March 3 2023 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