{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:54:51Z","timestamp":1772160891026,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"8-9","license":[{"start":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T00:00:00Z","timestamp":1727049600000},"content-version":"vor","delay-in-days":53,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100020374","name":"Ministry of AYUSH, Government of India","doi-asserted-by":"publisher","award":["Z.28015\/252\/2016\u2010HPC (EMR)\u2010AYUSH\u2010C"],"award-info":[{"award-number":["Z.28015\/252\/2016\u2010HPC (EMR)\u2010AYUSH\u2010C"]}],"id":[{"id":"10.13039\/100020374","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["RSPD2024R728"],"award-info":[{"award-number":["RSPD2024R728"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Obligate intracellular protozoan parasite,\n                    <jats:italic>Leishmania donovani<\/jats:italic>\n                    , causative agent of visceral leishmaniasis, led to impaired macrophage functions. It is well documented that many of these changes were induced by parasite\u2010mediated reduction in macrophage cholesterol content.\n                    <jats:italic>Leishmania<\/jats:italic>\n                    \u2010mediated alteration in the other lipids has not been explored in detail\u00a0yet. Here, we found that the expression of key cholesterol biosynthetic genes and total cellular cholesterol were reduced during\n                    <jats:italic>L. donovani<\/jats:italic>\n                    infection. Further, we have also identified that this reduction in the cholesterol led to increased membrane fluidity and inhibition of antigen\u2010presenting potential of macrophages. In addition to this, we studied the relative changes in different lipids in THP\u20101\u2010derived macrophages during\n                    <jats:italic>L. donovani<\/jats:italic>\n                    infection through liquid chromatography\u2010mass spectrometry. We found that Sphingomyelin (16:0) and ceramide (20:1, 26:0 and 26:1) were significantly reduced in infected macrophages. We further observed that the majority of different sub\u2010classes of phospholipids were downregulated significantly. Overall ratio of phosphatidylcholine versus phosphotidylethanolamine was decreased which indicated the compensatory mechanism of cell in response to cholesterol reduction. The observed\n                    <jats:italic>Leishmania<\/jats:italic>\n                    \u2010mediated alteration in macrophage\u2010lipidome provided the novel insights into mechanism of host\u2010pathogen interactions.\n                  <\/jats:p>","DOI":"10.1111\/pim.13066","type":"journal-article","created":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T05:59:29Z","timestamp":1727071169000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>Leishmania donovani<\/i>\n                    Modulates Macrophage Lipidome During Infection"],"prefix":"10.1111","volume":"46","author":[{"given":"Shams","family":"Tabrez","sequence":"first","affiliation":[{"name":"Infection and Immunity Lab (414), Department of Biotechnology Jamia Millia Islamia  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeeshan","family":"Fatima","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Amity University Gurgaon  Gurgaon India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sajjadul Kadir","family":"Akand","sequence":"additional","affiliation":[{"name":"Infection and Immunity Lab (414), Department of Biotechnology Jamia Millia Islamia  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Areeba","family":"Rahman","sequence":"additional","affiliation":[{"name":"Infection and Immunity Lab (414), Department of Biotechnology Jamia Millia Islamia  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saif","family":"Hameed","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Amity University Gurgaon  Gurgaon India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Saleem","sequence":"additional","affiliation":[{"name":"National Institute of Science Education and Research (NISER)  Bhubaneshwar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuf","family":"Akhter","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Baba Sahed Bhimrao Ambedkar University  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subhash Kumar","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Statistics Baba Sahed Bhimrao Ambedkar University  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Z.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yashwant","family":"Kumar","sequence":"additional","affiliation":[{"name":"Translational Health Science and Technology Institute  Faridabad 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