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The dense granule antigens (GRAs) have garnered crucial role in parasite survival, virulence and the formation of the parasitophorous vacuole. The study focusing on the GRA10 protein of <jats:italic>T. gondii<\/jats:italic> aims to elucidate its features further to support its potential inclusion in future vaccine formulations. The present study provided an exhaustive elucidation of the key characteristics of the GRA10 protein, encompassing its presence of a transmembrane domain, physico\u2010chemical properties, subcellular localization, potential epitopes recognised by B\u2010cells and T\u2010cells, secondary and tertiary structures, as well as other significant attributes of this protein. The results indicated that the GRA10 protein possesses a total of 192 possible post\u2010translational modification sites, with no transmembrane domains being detected within its structure. In terms of secondary structure, the GRA10 protein is composed of 27.74% alpha\u2010helix, 13.53% extended strand and 58.72% random coil elements. Additionally, various potential B\u2010 and T\u2010cell epitopes were pinpointed for the GRA10 protein, suggesting its immunogenic properties. The assessment of antigenicity and allergenicity further confirmed that GRA10 is immunogenic but non\u2010allergenic, making it a promising candidate for further study. Furthermore, the induction of IFN\u2010\u03b3 and IL\u20104 highlighted the ability of related MHC\u2010II molecules to interact with GRA10, indicating its potential role in immune responses. These findings shed light on the multifaceted nature of the GRA10 protein and its significance in immunological processes. The study presented crucial fundamental and theoretical information regarding GRA10 in order to facilitate the creation of a potent vaccine against both acute and chronic toxoplasmosis, warranting additional in\u00a0vivo examinations.<\/jats:p>","DOI":"10.1111\/pim.70004","type":"journal-article","created":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T11:55:22Z","timestamp":1740225322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>Toxoplasma gondii<\/i> (<scp>GRA10<\/scp>): A Closer Glance Into Immunogenic and Biochemical Characteristics Using In\u00a0Silico Approach"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8195-301X","authenticated-orcid":false,"given":"Mahdi","family":"Khadem Mohammadi","sequence":"first","affiliation":[{"name":"Student Research Committee, Faculty of Medicine Shahed University  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9635-2876","authenticated-orcid":false,"given":"Ali Dalir","family":"Ghaffari","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, Faculty of Medicine Shahed University  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00057\u201017"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182011001478"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines9050413"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05013-11"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00119-12"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.03.012"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(02)00256-4"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.06.017"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.03.003"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2014.52.6.581"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2019.01.005"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.01.040"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.2147\/ITT.S241064"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2020.03.006"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.04.033"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.09.016"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5SC03892H"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2010.06.004"},{"issue":"2","key":"e_1_2_10_20_1","first-page":"125","article-title":"DNA Vaccines and Recombinant Antigens in Prevention of Toxoplasma gondii Infections\u2014Current Status of the Studies","volume":"55","author":"Hiszczy\u0144ska\u2010Sawicka E.","year":"2009","journal-title":"Wiadomo\u015bci Parazytologiczne"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2019.105696"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2014.05.001"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-4"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq551"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl343"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp386"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-890-0:571"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep39787"},{"key":"e_1_2_10_29_1","volume-title":"Prediction of N\u2010Glycosylation Sites in Human Proteins. 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