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In this study, we describe the production and characterisation of IgY polyclonal antibodies capable of specifically recognising the SRS29B surface protein from\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    tachyzoites lysates. To this end, a series of bioinformatics tools were employed to predict potential SRS29B B\u2010cell epitopes, including Antheprot, IEDB, BCPREDS, ABCpred and Bepipred. Four peptides were synthesised to generate IgY antibodies. The production of specific antibodies against the SRS29B peptide constructs was confirmed in experimental animals using ELISA and MABA assays. This outcome demonstrated a correlation between the predictive analysis and the response obtained in chickens. Antibodies targeting the peptide constructs containing the SRS29B sequences (217\u2013229 aa, 238\u2013253 aa and 259\u2013274 aa) recognised molecules present in the tachyzoite lysates of\n                    <jats:italic>T.\u2009gondii<\/jats:italic>\n                    , as confirmed by western blot analysis. This specific recognition was further supported by the inhibition of tachyzoite protein recognition upon exposure to homologous peptides. These findings suggest that three selected peptide constructs are suitable for the development of an antigen\u2010capture assay to facilitate the detection of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    in raw milk as well as for diagnosing acute toxoplasmosis.\n                  <\/jats:p>","DOI":"10.1111\/pim.70063","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T04:32:02Z","timestamp":1769661122000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Generation and Characterisation of Peptide\u2010Based\n                    <scp>IgY<\/scp>\n                    Antibodies Against\n                    <scp>SRS29B<\/scp>\n                    Protein of\n                    <i>Toxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"48","author":[{"given":"Lauren Cruz","family":"Samaca","sequence":"first","affiliation":[{"name":"Fundaci\u00f3n Instituto de Estudios Avanzados (IDEA), Carretera Nacional Hoyo de la Puerta  Baruta Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noraida","family":"Zerpa","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto de Estudios Avanzados (IDEA), Carretera Nacional Hoyo de la Puerta  Baruta Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry","family":"Berm\u00fadez","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical, Universidad Central de Venezuela  Caracas Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oriana","family":"Zerpa","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto de Estudios Avanzados (IDEA), Carretera Nacional Hoyo de la Puerta  Baruta Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1922-902X","authenticated-orcid":false,"given":"Caridad","family":"Malav\u00e9","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto de Estudios Avanzados (IDEA), Carretera Nacional Hoyo de la Puerta  Baruta Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.04.003"},{"key":"e_1_2_11_3_1","first-page":"898","article-title":"Global Status of Toxoplasma gondii Infection: Systematic Review and Prevalence Snapshots","volume":"36","author":"Molan A.","year":"2019","journal-title":"Tropical 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