{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T07:41:44Z","timestamp":1777016504599,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["221914\/Z\/20\/Z"],"award-info":[{"award-number":["221914\/Z\/20\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["224014\/Z\/21\/Z"],"award-info":[{"award-number":["224014\/Z\/21\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n The IL\u201033\/ST2 pathway is important as part of the type 2 immune response against helminth infections. Mast cells express the highest levels of the IL\u201033 receptor subunit ST2 of any immune cell, and mast cells can mediate type 2 immune inflammation; however, the role of IL\u201033\u2010driven mast cell responses in helminth infection is poorly understood. We sought to determine the role of mast cell ST2 expression during\n Heligmosomoides polygyrus bakeri<\/jats:italic>\n (\n Hpb<\/jats:italic>\n ) infection by generating mast cell conditional ST2 knockout (MCPT5\n Cre<\/jats:sup>\n \u2009\u00d7\u2009ST2\n f\/f<\/jats:sup>\n , cKO) mice. These mice have normal frequencies of mast cells at steady state but show specific and strong (albeit incomplete) knockdown of ST2 expression on mast cells. On\n Hpb<\/jats:italic>\n infection, faecal egg and adult worm burden were similar between cKO and littermate controls, as were mast cell degranulation markers, serum IgE and goblet cell hyperplasia. Therefore, we conclude that mast cell ST2 does not play a dominant role in\n Hpb<\/jats:italic>\n infection. To further investigate the immune response to infection in cKO and littermate controls, transcriptomic and proteomic changes were assessed in duodenal tissues in infected versus na\u00efve mice in cKO and control mice. Minimal transcriptomic and proteomic changes were seen between genotypes, whereas substantial changes were seen between na\u00efve and infected mice, regardless of genotype.\n Hpb<\/jats:italic>\n infection induced local increases at the transcript and protein level for mast cell proteases (MCPT1 and MCPT2), resistin\u2010like molecules (RELM\u03b1 and RELM\u03b2) and markers such as the phospholipase PLA2G4C and the pore\u2010forming protein gasdermin C. Bulk proteomic analysis was also searched against the\n Hpb<\/jats:italic>\n genome to identify\n Hpb<\/jats:italic>\n proteins present in the duodenal tissues. A list of 60\n Hpb<\/jats:italic>\n proteins of interest was identified in infected duodenal samples, of which 18 contain a signal peptide and are present in the excretory\/secretory products of\n Hpb<\/jats:italic>\n (HES) (likely secretory products including immunomodulatory proteins); 28 proteins are present in HES but do not contain a signal peptide (likely excretory products); and 14 proteins are not present in HES (likely proteins present in the remnants of\n Hpb<\/jats:italic>\n within the duodenum). This work thus provides datasets for changes in the mouse intestine due to\n Hpb<\/jats:italic>\n infection, at both the transcript and protein level, as well as a dataset of\n Hpb<\/jats:italic>\n proteins detectable in the mouse duodenum at day 14 of infection.\n <\/jats:p>","DOI":"10.1111\/pim.70076","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T09:16:24Z","timestamp":1774862184000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic and Proteomic Analyses Show Minimal Role for\n ST2<\/scp>\n on\n MCPT5<\/scp>\n \u2010Expressing Mast Cells in Primary\n Heligmosomoides polygyrus bakeri<\/i>\n Infection"],"prefix":"10.1111","volume":"48","author":[{"given":"Nicole W. P.","family":"Ong","sequence":"first","affiliation":[{"name":"Division of Cell Signalling and Immunology, School of Life Sciences University of Dundee Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne H.","family":"Hodge","sequence":"additional","affiliation":[{"name":"Division of Cell Signalling and Immunology, School of Life Sciences University of Dundee Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christa P.","family":"Baker","sequence":"additional","affiliation":[{"name":"Division of Cell Signalling and Immunology, School of Life Sciences University of Dundee Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle J.","family":"Smyth","sequence":"additional","affiliation":[{"name":"Division of Cell Signalling and Immunology, School of Life Sciences University of Dundee Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tania","family":"Frangova","sequence":"additional","affiliation":[{"name":"Division of Cell Signalling and Immunology, School of Life Sciences University of Dundee Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry J.","family":"McSorley","sequence":"additional","affiliation":[{"name":"Division of Cell Signalling and Immunology, School of Life Sciences University of Dundee Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Global Health Estimates 2021: Disease Burden by Cause, Age, Sex, by Country and by Region, 2000\u20132021","author":"WHO","year":"2023"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281\u2010012\u20100347\u20103"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010023\u201000942\u20101"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010immunol\u2010032713\u2010120218"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.09.015"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.54017"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201001886\u20106"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2025.09.006"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.05.001"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2021.101532"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010023\u201001483\u20104"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010020\u20100687\u20104"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011\u2010009\u20100088\u20105"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.04.017"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2024.03.017"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248\u2010007\u20109153\u20104"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10163"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3791\/52412"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2025.115721"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.101725"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2011.06.002"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.3c00671"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010021\u201001156\u20103"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.02.043"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15269"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2026307118"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.07.003"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2016.56"},{"key":"e_1_2_9_32_1","doi-asserted-by":"crossref","unstructured":"M.Campillo Poveda O.Hardy R. F.Laidlaw T. D.Otto andR. M.Maizels \u201cSpatial Transcriptomics Reveals Recasting of Signalling Networks in the Small Intestine Following Tissue Invasion by the Helminth ParasiteHeligmosomoides polygyrus \u201d bioRxiv 2024.2002.2009.579622 2025 https:\/\/doi.org\/10.1101\/2024.02.09.579622.","DOI":"10.1101\/2024.02.09.579622"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.09.006"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010023\u201005785\u20102"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1038"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.70076","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pim.70076","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.70076","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T07:00:19Z","timestamp":1777014019000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.70076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,30]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1111\/pim.70076"],"URL":"https:\/\/doi.org\/10.1111\/pim.70076","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,30]]},"assertion":[{"value":"2025-12-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70076"}}