{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T06:58:24Z","timestamp":1785221904732,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271798"],"award-info":[{"award-number":["82271798"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301998"],"award-info":[{"award-number":["82301998"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515010849"],"award-info":[{"award-number":["2024A1515010849"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    \u03b3\u03b4T cells are a subset of innate lymphocytes that play an essential role in anti\u2010infection immunity. However, the functional role of CD8\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    \u03b3\u03b4T cells, a distinct subset of \u03b3\u03b4T cells, remains poorly characterized during malaria infection. Female C57BL\/6 mice were intraperitoneally injected with 1\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    <jats:italic>Plasmodium yoelii<\/jats:italic>\n                    \u2010infected red blood cells (iRBCs). At 12\u2009days post\u2010infection (12 dpi, the peak of parasitemia), spleens were harvested and lymphocytes were isolated. Fluorescence\u2010activated cell sorting (FACS) was performed to determine the frequency, phenotypic features and functional properties of CD8\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    \u03b3\u03b4T cells. Meanwhile, CD45\n                    <jats:sup>+<\/jats:sup>\n                    lymphocytes were sorted for single\u2010cell RNA sequencing (scRNA\u2010seq) to compare differentially expressed genes between CD8\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    \u03b3\u03b4T and CD8\u03b1\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    \u03b3\u03b4T cell subsets. FACS analysis revealed a significant increase in CD8\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    \u03b3\u03b4T cells following\n                    <jats:italic>P. yoelii<\/jats:italic>\n                    infection. These cells exhibited elevated expression of activation\u2010related molecules, enhanced effector functions, preferential polarization towards an IFN\u2010\u03b3\u2010producing (\u03b3\u03b4T1) phenotype, and reduced expression of exhaustion markers. scRNA\u2010seq further demonstrated that CD8\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    \u03b3\u03b4T cells upregulated genes involved in DNA replication and repair. Our findings indicate that CD8\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    \u03b3\u03b4T cells upregulate molecules associated with activation and function during\n                    <jats:italic>P. yoelii<\/jats:italic>\n                    infection, suggesting that they may play a more prominent role in host defence against malaria.\n                  <\/jats:p>","DOI":"10.1111\/pim.70091","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:10:22Z","timestamp":1783008622000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characteristics of Splenic\n                    <scp>CD8\u03b1\u03b2<\/scp>\n                    <sup>+<\/sup>\n                    <scp>\u03b3\u03b4T<\/scp>\n                    Cells in\n                    <i>Plasmodium yoelii<\/i>\n                    Infection"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4715-6865","authenticated-orcid":false,"given":"Xingfei","family":"Pan","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Key Laboratory for Major Obstetric Diseases of Guangdong Province The Third Affiliated Hospital, Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Key Laboratory for Major Obstetric Diseases of Guangdong Province The Third Affiliated Hospital, Guangzhou Medical University  Guangzhou China"},{"name":"Laboratory of Immunology, School of Basic Medicine Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyue","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Key Laboratory for Major Obstetric Diseases of Guangdong Province The Third Affiliated Hospital, Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Key Laboratory for Major Obstetric Diseases of Guangdong Province The Third Affiliated Hospital, Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiwen","family":"Yuan","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, School of Basic Medicine Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjie","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, School of Basic Medicine Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiongyu","family":"Xie","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, School of Basic Medicine Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Ye","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, School of Basic Medicine Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixin","family":"Luo","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, School of Basic Medicine Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guikuan","family":"Liang","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, School of Basic Medicine Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Xie","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, School of Basic Medicine Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6135-1256","authenticated-orcid":false,"given":"Jun","family":"Huang","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, School of Basic Medicine Guangzhou Medical University  Guangzhou China"},{"name":"Department of Laboratory Medicine The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital  Qingyuan China"},{"name":"Guangdong Provincial Key Laboratory of Allergy and Clinical Immunology, the Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy and Clinical Immunology Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-021-03619-z"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007440"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.788546"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-27339-8"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.6.689"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1312872111"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jleuko\/qiad131"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.4.537"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1814440116"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.abc5884"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-023-00542-2"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.10.1485"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201243231"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2023020528"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.adk4348"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.ade3525"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-022-01161-x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-024-01855-4"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601146"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202400024"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2023.2221485"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903529"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-05-352732"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2326-y"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(83)90049-4"},{"issue":"31","key":"e_1_2_10_27_1","first-page":"E374","article-title":"Linkage Maps From Multiple Genetic Crosses and Loci Linked to Growth\u2010Related Virulent Phenotype in Plasmodium yoelii","volume":"108","author":"Li J.","year":"2011","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-023-09441-w"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-023-07950-z"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1123074"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay3224"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.582358"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abm9120"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06231-0"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201646875"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2022.02.005"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.70091","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pim.70091","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.70091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:59:41Z","timestamp":1785218381000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.70091"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":35,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/pim.70091"],"URL":"https:\/\/doi.org\/10.1111\/pim.70091","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70091"}}