{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T06:58:25Z","timestamp":1785221905805,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Infection with the intracellular apicomplexan parasite\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    causes severe and often fatal clinical outcomes worldwide, especially in patients with immunodeficiency, diabetes and in pregnant women and infants. Despite approximately one\u2010third of the global population being infected with\n                    <jats:italic>T. gondii<\/jats:italic>\n                    , there is currently no effective vaccine available for humans. The objective was to develop a potential vaccine candidate for\n                    <jats:italic>T. gondii<\/jats:italic>\n                    , which would incorporate the B\u2010 and T\u2010lymphocyte epitopes derived from three immunogenic antigens of the parasite. Initially, the immunodominant epitopes present in the SAG1, GRA6 and GRA7 proteins of\n                    <jats:italic>T. gondii were<\/jats:italic>\n                    identified. Following this, a multi\u2010epitope vaccine was developed by integrating B\u2010cell epitopes, CTL epitopes and HTL epitopes, with the addition of the 50S ribosomal protein L7\/L12 serving as an adjuvant to enhance the immunogenic properties of the vaccine. All identified epitopes demonstrated characteristics of being antigenic, nonallergenic, nontoxic and lacking human homologues. Furthermore, the candidate vaccine exhibited immunogenicity, non\u2010allergenicity and stability. Molecular docking studies indicated robust interactions between the vaccine construct and the TLR\u20104 immune receptor. Additionally, the stability of the formulated vaccine was confirmed through molecular dynamic simulations. In silico analyses suggested that the vaccine construct could effectively initiate primary immune responses; however, further laboratory evaluations are required to verify its efficacy and safety.\n                  <\/jats:p>","DOI":"10.1111\/pim.70095","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:37:24Z","timestamp":1784767044000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Immunoinformatic Approach for a Multi\u2010Epitope Vaccine Against\n                    <scp>\n                      <i>Toxoplasma gondii<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"48","author":[{"given":"Negar","family":"Asadi","sequence":"first","affiliation":[{"name":"Student Research Committee Urmia University of Medical Sciences  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Navapour","sequence":"additional","affiliation":[{"name":"Biophysics and Computational Biology Laboratory (BCBL), Department of Biology, College of Sciences Shiraz University  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navid","family":"Mogharrab","sequence":"additional","affiliation":[{"name":"Biophysics and Computational Biology Laboratory (BCBL), Department of Biology, College of Sciences Shiraz University  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elham","family":"Yousefi","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute Urmia University of Medical Sciences  Urmia Iran"},{"name":"Department of Medical Parasitology and Mycology, School of Medicine Urmia University of Medical Sciences  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadegh","family":"Feizollahzadeh","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute Urmia University of Medical Sciences  Urmia Iran"},{"name":"Department of Immunology and Genetics, School of Medicine Urmia University of Medical Sciences  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9659-7491","authenticated-orcid":false,"given":"Mortaza","family":"Taheri\u2010Anganeh","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute Urmia University of Medical Sciences  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahram","family":"Khademvatan","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute Urmia University of Medical Sciences  Urmia Iran"},{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science The University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon S.","family":"Howarth","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide  Roseworthy South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"issue":"2","key":"e_1_2_11_2_1","first-page":"193","article-title":"Association of Toxoplasma gondii Infection With Cardiovascular Diseases: A Cross\u2010Sectional Study Among Patients With Heart Failure Diseases in Urmia, North\u2010West of Iran","volume":"66","author":"Khademvatan S.","year":"2020","journal-title":"Annals of 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