{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:01:57Z","timestamp":1790161317034,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Disseminated acanthamoebiasis is a rare but severe opportunistic infection that can involve multiple organs, including the liver, causing fatal outcomes. This study provides new insights into hepatic pathogenesis and possible relationship with angiogenesis activity and the therapeutic role of quercetin conjugated silver nanoparticles (Q\u2010AgNPs). Sixty BALB\/c mice were divided into four groups: uninfected control, uninfected treated with Q\u2010AgNPs, infected group and infected treated with Q\u2010AgNPs. The Q\u2010AgNPs were synthesised and characterised by spectrophotometry, electron microscope, zeta potential and dynamic light scattering. Hepatic tissues were examined histopathologically for granulomas (classified as caseating\/non\u2010caseating and immature\/mature) and vascular changes. Hypoglycaemia and expression of angiogenic markers (CD31, CD34 and MMP9) were assessed.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Acanthamoeba polyphaga<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection induced significant hepatic granulomatous inflammation, hypoglycaemia, vascular remodelling and upregulation of CD31, CD34 and MMP9, indicating active angiogenesis. Granulomas exhibited necrotic cores in some cases and progressed from immature to mature stages. Q\u2010AgNPs treatment markedly attenuated granuloma numbers, suppressing angiogenic marker expression, mitigating inflammation, capillary permeability and liver lesions. This study provides novel evidence linking\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    infection to hepatic granuloma formation and angiogenesis\u2010driven pathology which may serve as useful biomarkers for extracerebral acanthamoebiasis. Further studies are needed to evaluate long\u2010term outcomes and host\u2013parasite interaction.\n                  <\/jats:p>","DOI":"10.1111\/pim.70098","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T03:30:41Z","timestamp":1788492641000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hepatic Granulomatous Inflammation and Angiogenesis Upregulation Provoked by\n                    <i>Acanthamoeba polyphaga<\/i>\n                    Infection and Role of Quercetin\u2010Conjugated Silver Nanoparticles"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2638-624X","authenticated-orcid":false,"given":"Yousef Abdal Jalil","family":"Fadladdin","sequence":"first","affiliation":[{"name":"Faculty of Sciences, Department of Biological Sciences King Abdulaziz University  Jeddah Saudi 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