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Here, we characterized acute infection outcomes and myocardial innate immune transcriptional profiles in mice subjected to single and mixed infections with genetically distinct\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T. cruzi<\/jats:italic>\n                    <\/jats:styled-content>\n                    parasites (DTUs TcII, TcIII, and TcVI). Swiss mice were infected with RN2, RN23, Y, or CL\u2010Brener individually or in combination, and parasitemia, survival, and cardiac gene expression were assessed. The Y strain, CL\u2010Brener clone, and RN2\u2009+\u2009RN23\u2009+\u2009CL\u2010Brener condition exhibited a high\u2010severity profile, with marked parasitemia (8000\u201328,000 trypomastigotes\/5\u2009\u03bcL), mortality of 74%\u2013100%, reduced myocardial transcription of\n                    <jats:italic>Tlr2<\/jats:italic>\n                    ,\n                    <jats:italic>Tlr4<\/jats:italic>\n                    ,\n                    <jats:italic>Tlr9<\/jats:italic>\n                    ,\n                    <jats:italic>Trif<\/jats:italic>\n                    , and\n                    <jats:italic>Myd88<\/jats:italic>\n                    , and increased transcription of\n                    <jats:italic>Nlrp3<\/jats:italic>\n                    ,\n                    <jats:italic>Casp1<\/jats:italic>\n                    ,\n                    <jats:italic>Il1\u03b2<\/jats:italic>\n                    , and\n                    <jats:italic>Tnf\u03b1<\/jats:italic>\n                    . In contrast, RN2 and RN23 single infections exhibited low\u2010severity profiles and higher transcription of innate immune mediators associated with parasite control. Mixed\u2010infection groups displayed transcriptional and infection profiles differing from the corresponding single\u2010infection groups under a standardized total inoculum. Together, these findings identify distinct myocardial innate immune transcriptional profiles across single and mixed\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T. cruzi<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection conditions and support further investigation of the contributions of parasite population composition and dose to acute infection outcomes.\n                  <\/jats:p>","DOI":"10.1111\/pim.70103","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:11:43Z","timestamp":1791360703000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous Infection With Genetically Distinct\n                    <i>Trypanosoma cruzi<\/i>\n                    Isolates Is Associated With Distinct Myocardial Innate Immune Transcriptional Profiles During Acute Infection in Mice"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9525-6532","authenticated-orcid":false,"given":"George Harisson Felinto","family":"Sampaio","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Centro de Ci\u00eancias da Sa\u00fade Universidade Federal do Rio Grande do Norte (UFRN)  Natal Rio Grande do Norte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6584-5771","authenticated-orcid":false,"given":"Andressa Noronha","family":"Barbosa\u2010Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Biologia Parasit\u00e1ria Centro de Bioci\u00eancias, UFRN  Natal Rio Grande do Norte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4882-5215","authenticated-orcid":false,"given":"Carlos Ramon","family":"do Nascimento 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Parasitologia Centro de Bioci\u00eancias, UFRN  Natal Rio Grande do Norte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7337-9320","authenticated-orcid":false,"given":"Antonia Claudia J\u00e1come","family":"da C\u00e2mara","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Clinicas e Toxicol\u00f3gicas Centro de Ci\u00eancias da Sa\u00fade, UFRN  Natal Rio Grande do Norte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4564-6791","authenticated-orcid":false,"given":"Paulo Marcos","family":"da Matta Guedes","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia e Parasitologia Centro de Bioci\u00eancias, UFRN  Natal Rio Grande do Norte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6207-4502","authenticated-orcid":false,"given":"L\u00facia Maria","family":"da Cunha 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