{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T17:17:38Z","timestamp":1763140658014},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T00:00:00Z","timestamp":1376956800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>Embryonic stem (<jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content>) cells deficient in poly(<jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010ribose) polymerase\u20101 (<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101) develop into teratocarcinomas with the appearance of trophoblast giant cells (<jats:styled-content style=\"fixed-case\">TGCs<\/jats:styled-content>) when injected subcutaneously into nude mice. Because the uterus is one of the original organs in which germ cell tumors develop with induction of trophoblast lineage, here we investigated whether <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic> deficiency in <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> cells affects teratocarcinoma formation processes by grafting <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> cells into the horns of uteri. Teratocarcinomas developed from both wild\u2010type (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic><jats:sup>+\/+<\/jats:sup>) and <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> cells. The weights of the tumors derived from <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> cells were lower than those of the tumors derived from <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic><jats:sup>+\/+<\/jats:sup> <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> cells (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> tumors showed the appearance of <jats:styled-content style=\"fixed-case\">TGCs<\/jats:styled-content>. Notably, organ metastasis to the lung and liver was observed for the <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> tumors, but not for the <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic><jats:sup>+\/+<\/jats:sup> tumors (<jats:italic>P<\/jats:italic> &lt; 0.05). Invasions were more frequently observed with the <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> tumors compared with the <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic><jats:sup>+\/+<\/jats:sup> tumors (<jats:italic>P<\/jats:italic> &lt; 0.05). Since <jats:styled-content style=\"fixed-case\">TGCs<\/jats:styled-content> are known to have invasive properties, the appearance of <jats:styled-content style=\"fixed-case\">TGCs<\/jats:styled-content> may have supported the metastatic process. The present findings suggest that loss of <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arp\u20101<\/jats:italic> during teratocarcinoma formation might augment invasive and metastatic properties of the tumors in the uterine environment.<\/jats:p>","DOI":"10.1111\/pin.12086","type":"journal-article","created":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T10:05:19Z","timestamp":1376993119000},"page":"408-414","source":"Crossref","is-referenced-by-count":9,"title":["<i>Parp\u20101<\/i> deficiency in <scp>ES<\/scp> cells promotes invasive and metastatic lesions accompanying induction of trophoblast giant cells during tumorigenesis in uterine environment"],"prefix":"10.1111","volume":"63","author":[{"given":"Tadashige","family":"Nozaki","sequence":"first","affiliation":[{"name":"Division of Biochemistry National Cancer Center Research Institute  Tokyo Japan"},{"name":"Department of Pharmacology Osaka Dental University  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Fujimori","sequence":"additional","affiliation":[{"name":"Division of Biochemistry National Cancer Center Research Institute  Tokyo Japan"},{"name":"Division of Genome Stability Research National Cancer Center Research Institute  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhui","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Genome Stability Research National Cancer Center Research Institute  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Research Unit for Functional Genomics, National Research Center for Protozoan Diseases Obihiro University of Agriculture and Veterinary Medicine  Obihiro Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Imai","sequence":"additional","affiliation":[{"name":"Pathology Division Mie University Hospital  Mie Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Laboratory for Medical Engineering Division of Materials Science and Chemical Engineering Graduate School of Engineering Yokohama National University  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoshi","family":"Ohura","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Osaka Dental University  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuko","family":"Masutani","sequence":"additional","affiliation":[{"name":"Division of Biochemistry National Cancer Center Research Institute  Tokyo Japan"},{"name":"Division of Genome Stability Research National Cancer Center Research Institute  Tokyo Japan"},{"name":"ADP\u2010Ribosylation in Oncology Project National Cancer Center Research Institute  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-004-4509-x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1963"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.5.2301"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10250"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006941016799"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2007.00567.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.11.002"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1149250"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01314-07"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.23.13345"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00097-6"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-8-41"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(01)00703-1"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910560317"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1356-9597.2004.00736.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijdevneu.2009.04.002"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.429134"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.2.260"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.9073"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7527157"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.372557"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606528103"}],"container-title":["Pathology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpin.12086","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pin.12086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T20:35:18Z","timestamp":1696192518000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pin.12086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1111\/pin.12086"],"URL":"https:\/\/doi.org\/10.1111\/pin.12086","archive":["Portico"],"relation":{},"ISSN":["1320-5463","1440-1827"],"issn-type":[{"value":"1320-5463","type":"print"},{"value":"1440-1827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8]]}}}