{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T12:32:02Z","timestamp":1766579522394},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,8,22]],"date-time":"2014-08-22T00:00:00Z","timestamp":1408665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>irschsprung disease (<jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>) is a congenital disease resulting from failure of neural crest\u2010derived ganglion cells to colonize the colon. Conventional diagnostic methods are insufficient for evaluating the \u2018functional\u2019 prognosis of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. In order to elucidate the maturation of ganglion cells, 17 immunohistochemical markers were examined. We examined the digestive tracts of 2 human early delivery patients, 2 miniature swine fetuses, 4 little infants, 3 infants, 3 children, 6 adults, and 3 aged individuals. With increasing age, the labeling index (<jats:styled-content style=\"fixed-case\">LI<\/jats:styled-content>) for both calretinin and tyrosine hydroxylase (<jats:styled-content style=\"fixed-case\">TH<\/jats:styled-content>) increased, whereas that for <jats:styled-content style=\"fixed-case\">SOX<\/jats:styled-content>10 decreased. We then examined the \u2018transitional zone\u2019 of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> in 21 affected patients and 18 controls for these three markers. The <jats:styled-content style=\"fixed-case\">LI<\/jats:styled-content> of calretinin and <jats:styled-content style=\"fixed-case\">TH<\/jats:styled-content> were significantly lower than in the controls (median: 3.7 in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> and 8.2 in controls, <jats:italic>P<\/jats:italic> &lt; 0.001, median: 27.9 in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> and 44.4 in controls, <jats:italic>P<\/jats:italic> &lt; 0.001, respectively). In contrast, the <jats:styled-content style=\"fixed-case\">LI<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">SOX<\/jats:styled-content>10 showed no significant difference (median: 33.7 in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> and 29.2 in controls, <jats:italic>P<\/jats:italic> = 0.666) however, hierarchical cluster analysis was able to divide <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> patients into two groups. These results suggest that immature ganglion cells are present in the transitional zone of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>, and that <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> may have two different pathophysiological processes.<\/jats:p>","DOI":"10.1111\/pin.12191","type":"journal-article","created":{"date-parts":[[2014,8,22]],"date-time":"2014-08-22T09:25:10Z","timestamp":1408699510000},"page":"432-442","source":"Crossref","is-referenced-by-count":8,"title":["Developmental markers of ganglion cells in the enteric nervous system and their application for evaluation of <scp>H<\/scp>irschsprung disease"],"prefix":"10.1111","volume":"64","author":[{"given":"Hitomi","family":"Kawai","sequence":"first","affiliation":[{"name":"Department of Pathology Tsukuba University Hospital University of Tsukuba  Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaishi","family":"Satomi","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Faculty of Medicine University of Tsukuba  Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Morishita","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Tokyo Medical University Ibaraki Medical Center  Ami Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Murata","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Graduate School of Comprehensive Human Sciences University of Tsukuba  Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Sugano","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Faculty of Medicine University of Tsukuba  Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyuki","family":"Nakano","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Graduate School of Comprehensive Human Sciences University of Tsukuba  Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Faculty of Medicine University of Tsukuba  Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,8,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2012.32"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901010212"},{"key":"e_1_2_6_4_1","first-page":"1","article-title":"Stuhltr\u00e4gheit Neugeborener in Folge von Dilatation und Hypertrophie des Colons","volume":"27","author":"Hirschsprung 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