{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T08:14:44Z","timestamp":1782634484412,"version":"3.54.5"},"reference-count":13,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,9,27]],"date-time":"2016-09-27T00:00:00Z","timestamp":1474934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>Detection of HTLV\u20101 provirus using paraffin tumor sections may assist the diagnosis of adult T\u2010cell leukemia\/lymphoma (ATLL). For the detection, non\u2010quantitative PCR assay has been reported, but its usefulness and limitations remain unclear. To our knowledge, quantitative PCR assay using paraffin tumor sections has not been reported. Using paraffin sections from ATLLs and non\u2010ATLL T\u2010cell lymphomas, we first performed non\u2010quantitative PCR for HTLV\u20101 provirus. Next, we determined tumor ratios and carried out quantitative PCR to obtain provirus copy numbers. The results were analyzed with a simple regression model and a novel criterion, cut\u2010off using 95 % rejection limits. Our quantitative PCR assay showed an excellent association between tumor ratios and the copy numbers (<jats:italic>r<\/jats:italic> = 0.89, <jats:italic>P<\/jats:italic> &lt; 0.0001). The 95 % rejection limits provided a statistical basis for the range for the determination of HTLV\u20101 involvement. Its application suggested that results of non\u2010quantitative PCR assay should be interpreted very carefully and that our quantitative PCR assay is useful to estimate the status of HTLV\u20101 involvement in the tumor cases. In conclusion, our quantitative PCR assay using paraffin tumor sections may be useful for the screening of ATLL cases, especially in HTLV\u20101 non\u2010endemic areas where easy access to serological testing for HTLV\u20101 infection is limited.<\/jats:p>","DOI":"10.1111\/pin.12462","type":"journal-article","created":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T00:17:02Z","timestamp":1475021822000},"page":"618-621","source":"Crossref","is-referenced-by-count":4,"title":["Quantitative PCR for HTLV\u20101 provirus in adult T\u2010cell leukemia\/lymphoma using paraffin tumor sections"],"prefix":"10.1111","volume":"66","author":[{"given":"Junki","family":"Kato","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Nagoya City University Graduate School of Medical Sciences  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayako","family":"Masaki","sequence":"additional","affiliation":[{"name":"Department of 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