{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T16:58:01Z","timestamp":1783184281075,"version":"3.54.6"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,27]],"date-time":"2016-11-27T00:00:00Z","timestamp":1480204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cervical cancer screening based on the Papanicolaou (Pap) test is a widely applied but not always efficient practice for detecting Human Papillomavirus (HPV) mediated lesions, partially due to a non\u2010systematic and inadequate screening process. Our aim was to introduce an inexpensive easy\u2010to\u2010use direct screening platform for improved detection of abnormal cells indicative of underlying cervical neoplasia as well as persisting HPV infection. By employing a novel, efficient technique of laser\u2010based micromachining, we achieved the fabrication of spatial grids on commercially available coverslips allowing visual segmentation of the slide for efficient screening. Abnormal and formerly diagnosed as negative for intraepithelial lesion or malignancy (NILM) Pap test slides (<jats:italic>n<\/jats:italic> = 200) were analyzed by conventional and grid\u2010based screening. Grid\u2010based microscopy led to a more reliable diagnosis compared to the conventional by identifying an increased number of abnormal cells (<jats:italic>P<\/jats:italic> = 0.001). It decreased borderline ASCUS, AGC diagnosis, increasing LSIL, HSIL and <jats:italic>in situ<\/jats:italic> AdenoCa detection rates closely related with biopsy (<jats:italic>P<\/jats:italic> = 0.015; kappa = 0.978). Concerning the set of NILM diagnoses in rapid re\u2010screening, the method upgraded six cases (<jats:italic>n<\/jats:italic> = 6) to LSIL (<jats:italic>P<\/jats:italic> = 0.001). The proposed technical solution offers a calibration and orientation visual aid during the on\u2010site screening process providing significant advantages compared to expensive digital imaging techniques.<\/jats:p>","DOI":"10.1111\/pin.12481","type":"journal-article","created":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T03:42:39Z","timestamp":1480304559000},"page":"24-31","source":"Crossref","is-referenced-by-count":3,"title":["Implementation of a real\u2010time reference and calibration grid platform for improved screening \u2013 mapping in Pap test slides"],"prefix":"10.1111","volume":"67","author":[{"given":"Evangelos","family":"Tsiambas","sequence":"first","affiliation":[{"name":"Department of Cytology &amp; Digital Image Analysis MycoLab  Athens Greece"},{"name":"Department of Molecular Biology &amp; IHC 401 General Military Hospital  Athens Greece"},{"name":"Department of Pathology, Medical School National and Kapodistrian University of Athens  Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos","family":"Riziotis","sequence":"additional","affiliation":[{"name":"Theoretical and Physical Chemistry Institute, Photonics for Nanoapplications Laboratory National Hellenic Research Foundation  Athens 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