{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T06:57:48Z","timestamp":1777705068501,"version":"3.51.4"},"reference-count":8,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,7,22]],"date-time":"2017-07-22T00:00:00Z","timestamp":1500681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:sec><jats:label\/><jats:p>Fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) is an essential tool for genetic diagnosis in daily pathological work. Almost full automation of FISH can be achieved with the recently released automated SureFISH platform (Dako Omnis, Agilent Technologies, Santa Clara, CA, USA). Its utility has been reported in <jats:italic>HER2<\/jats:italic> amplification of breast and gastric carcinoma and <jats:italic>ALK\u2010<\/jats:italic>rearranged lung cancer. Here, we examined the utility of automated SureFISH for the identification of rearrangement signals in translocation\u2010related sarcomas (TRSs), including 11 <jats:italic>EWSR1<\/jats:italic>\u2010rearranged and 10 synovial sarcoma cases, compared with non\u2010automated conventional FISH using the same specimens. The percentages of <jats:italic>EWSR1<\/jats:italic> or <jats:italic>SS18<\/jats:italic> split signals were higher in automated SureFISH than in conventional FISH in 13 of the 21 cases. On the other hand, 8 of the 21 cases showed the same or lower percentage of split signals in automated SureFISH. Both FISH approaches detected <jats:italic>EWSR1<\/jats:italic> and <jats:italic>SS18<\/jats:italic> split signals in more than 10% of tumor cells in all cases. The strongest advantage of automated SureFISH is its ability to reduce running time without sacrificing quality. Other advantages include improved signal sharpness with oligo probes and reduced ecological toxicity by avoiding formamide use. Automated SureFISH is an excellent tool for the genetic diagnosis of TRSs and contributes to their rapid definitive diagnosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pin.12562","type":"journal-article","created":{"date-parts":[[2017,7,23]],"date-time":"2017-07-23T04:32:41Z","timestamp":1500784361000},"page":"510-513","source":"Crossref","is-referenced-by-count":4,"title":["Diagnostic utility of automated SureFISH (Dako Omnis) in the diagnosis of musculoskeletal translocation\u2010related sarcomas"],"prefix":"10.1111","volume":"67","author":[{"given":"Shintaro","family":"Sugita","sequence":"first","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Aoyama","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimasa","family":"Ito","sequence":"additional","affiliation":[{"name":"Diagnostic and Genomics Group Japan Agilent Technologies Japan, Ltd. Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroko","family":"Asanuma","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taro","family":"Sugawara","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Segawa","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumika","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriaki","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuhiro","family":"Tsujiwaki","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromi","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadashi","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jos.2017.02.004"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-016-0486-2"},{"key":"e_1_2_6_4_1","first-page":"1327","article-title":"Analysis of HER2 status in gastroesophageal tumor specimens using a new automated HER2 IQFISH pharmDx\u2122 (Dako Omnis) assay","volume":"31","author":"Viale G","year":"2016","journal-title":"Histol Histopathol"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2016.06.002"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2016-204311"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminoncol.2009.06.004"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2011.02739.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040675"}],"container-title":["Pathology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpin.12562","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pin.12562","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T13:21:15Z","timestamp":1695993675000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pin.12562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,22]]},"references-count":8,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1111\/pin.12562"],"URL":"https:\/\/doi.org\/10.1111\/pin.12562","archive":["Portico"],"relation":{},"ISSN":["1320-5463","1440-1827"],"issn-type":[{"value":"1320-5463","type":"print"},{"value":"1440-1827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,22]]}}}