{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:24:17Z","timestamp":1762298657967,"version":"3.37.3"},"reference-count":21,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,9,3]],"date-time":"2017-09-03T00:00:00Z","timestamp":1504396800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:label\/><jats:p>The BIOMED\u20102 PCR protocol for targeting the <jats:italic>IGH<\/jats:italic> gene is widely employed for detecting clonality in B\u2010cell malignancies. Unfortunately, the detection of clonality with this method is not very sensitive when paraffin sections are used as a DNA source. To increase the sensitivity, we devised a semi\u2010nested modification of a <jats:italic>JH<\/jats:italic> consensus primer. The clonality detection rates of three assays were compared: the standard BIOMED\u20102, BIOMED\u20102 assay followed by BIOMED\u20102 re\u2010amplification, and BIOMED\u20102 assay followed by semi\u2010nested BIOMED\u20102. We tested more than 100 cases using paraffin\u2010embedded tissues of various B\u2010cell lymphomas, and found that the clonality detection rates with the above three assays were 63.9%, 79.6%, and 88.0%, respectively. While BIOMED\u20102 re\u2010amplification was significantly more sensitive than the standard BIOMED\u20102, the semi\u2010nested BIOMED\u20102 was significantly more sensitive than both the standard BIOMED\u20102 and BIOMED\u20102 re\u2010amplification. An increase in sensitivity was observed in all lymphoma subtypes examined. In conclusion, tumor clonality may be detected in nearly 90% of B\u2010cell lymphoma cases with semi\u2010nested BIOMED\u20102. This ancillary assay may be useful when the standard BIOMED\u20102 fails to detect clonality in histopathologically suspected B\u2010cell lymphomas.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pin.12566","type":"journal-article","created":{"date-parts":[[2017,9,4]],"date-time":"2017-09-04T04:15:20Z","timestamp":1504498520000},"page":"453-460","source":"Crossref","is-referenced-by-count":6,"title":["Improved clonality detection in B\u2010cell lymphoma using a semi\u2010nested modification of the BIOMED\u20102 PCR assay for <i>IGH<\/i> rearrangement: A paraffin\u2010embedded tissue study"],"prefix":"10.1111","volume":"67","author":[{"given":"Yuma","family":"Sakamoto","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Masaki","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Nagoya 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