{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T02:16:41Z","timestamp":1784168201718,"version":"3.55.0"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:label\/><jats:p>The BIOMED\u20102 PCR protocols targeting <jats:italic>IGH<\/jats:italic> and <jats:italic>IGK<\/jats:italic> genes may be useful for detecting clonality in Hodgkin lymphoma (HL). The clonality detection rates, however, have not been very high with these methods using paraffin\u2010embedded tumor sections. We previously described the usefulness of the semi\u2010nested BIOMED\u20102 <jats:italic>IGH<\/jats:italic> assay in B\u2010cell malignancies. In this study, we devised a novel semi\u2010nested BIOMED\u20102 <jats:italic>IGK<\/jats:italic> assay. Employing 58 cases of classical HL, we carried out the standard BIOMED\u20102, BIOMED\u20102 followed by BIOMED\u20102 re\u2010amplification, and BIOMED\u20102 followed by semi\u2010nested BIOMED\u20102, all targeting <jats:italic>IGH<\/jats:italic> and <jats:italic>IGK<\/jats:italic>, using paraffin\u2010embedded tissues. In both <jats:italic>IGH<\/jats:italic> and <jats:italic>IGK<\/jats:italic> assays, semi\u2010nested assays yielded significantly higher clonality detection rates than the standard assays and re\u2010amplification assays. Clonality was detected in 13\/58 (22.4%) classical HL cases using the standard <jats:italic>IGH\/IGK<\/jats:italic> assays while it was detected in 38\/58 (65.5%) cases using semi\u2010nested <jats:italic>IGH\/IGK<\/jats:italic> assays. The detection rates were not associated with the HL subtypes, CD30\u2010positive cell density, CD20\u2010positive cell density, or Epstein\u2013Barr virus (EBV) positivity. In conclusion, tumor clonality was detected in nearly two\u2010thirds of classical HL cases using semi\u2010nested BIOMED\u20102 <jats:italic>IGH\/IGK<\/jats:italic> assays using paraffin tumor sections. These semi\u2010nested assays may be useful when the standard <jats:italic>IGH\/IGK<\/jats:italic> assays fail to detect clonality in histopathologically suspected HLs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pin.12660","type":"journal-article","created":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T16:48:17Z","timestamp":1521996497000},"page":"287-293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Improved clonality detection in Hodgkin lymphoma using a semi\u2010nested modification of the BIOMED\u20102 PCR assay for <i>IGH<\/i> and <i>IGK<\/i> rearrangements: A paraffin\u2010embedded tissue study"],"prefix":"10.1111","volume":"68","author":[{"given":"Shusen","family":"Han","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Nagoya City University Graduate School of Medical Sciences Nagoya 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