{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T04:14:04Z","timestamp":1785039244404,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,6,4]],"date-time":"2018-06-04T00:00:00Z","timestamp":1528070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:sec><jats:label\/><jats:p>Granulomatous mastitis (GM) is a rare inflammatory disease of the post\u2010lactation breast, clinically mimicking breast cancer. GM is microscopically characterized by formation of epithelioid granulomas and abscess (suppurative granulomas) with lipid droplet\u2010centered inflammation. <jats:italic>Corynebacterium kroppenstedtii<\/jats:italic> (Ck) is known as a causative bacterium of GM, and identification of Ck infection within the lesion should thus be essential for confirming the diagnosis. In the present study, we analyzed formalin\u2010fixed, paraffin\u2010embedded (FFPE) biopsy specimens of a total of 18 GM lesions with immunostaining and real\u2010time PCR for Ck genome. Widely cross\u2010reactive rabbit antisera against Bacillus Calmette\u2010Guerin (BCG), <jats:italic>Bacillus cereus<\/jats:italic>, <jats:italic>Treponema pallidum<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic> were chosen. With real\u2010time PCR, Ck genome was demonstrated in 7 of 18 GM lesions. Immunohistochemically, the low\u2010specificity antisera reacted with the cytoplasm of phagocytes and\/or granuloma\u2010engulfed lipid droplets in 12 of 18 GM lesions. Antigenic positivity was observed in the following order: BCG &gt; <jats:italic>B. cereus<\/jats:italic> &gt; <jats:italic>T. pallidum<\/jats:italic> &gt; <jats:italic>E. coli<\/jats:italic>. Real\u2010time PCR using DNA extracted from FFPE sections was useful but not consistent for identifying the Ck genome in GM, while immunostaining using cross\u2010reactive antisera against four kinds of bacteria was not Ck\u2010specific but was applicable to visualizing bacterial infection within the GM lesions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pin.12683","type":"journal-article","created":{"date-parts":[[2018,6,4]],"date-time":"2018-06-04T09:01:31Z","timestamp":1528102891000},"page":"409-418","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["<i>Corynebacterium kroppenstedtii<\/i> in granulomatous mastitis: Analysis of formalin\u2010fixed, paraffin\u2010embedded biopsy specimens by immunostaining using low\u2010specificity bacterial antisera and real\u2010time polymerase chain reaction"],"prefix":"10.1111","volume":"68","author":[{"given":"Mari","family":"Fujii","sequence":"first","affiliation":[{"name":"Medical student"},{"name":"Department of Pathology Fujita Health University School of Medicine Toyoake"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyoshi","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Department of Pathology Fujita Health University School of Medicine Toyoake"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiko","family":"Sakuma","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouichiro","family":"Tagami","sequence":"additional","affiliation":[{"name":"Surgery Atsumi Hospital Tahara"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiichiro","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Surgery Toyohashi Medical Center Toyohashi Aichi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Kuno","sequence":"additional","affiliation":[{"name":"Hinaga Gastrointestinal and Breast Clinic Yokkaichi Mie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michihiko","family":"Harada","sequence":"additional","affiliation":[{"name":"Minami\u2010Nagano Koen Clinic Nagano"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Kubouchi","sequence":"additional","affiliation":[{"name":"Yokohama Breast and GI Clinic Kohoku\u2010ku Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Tsutsumi","sequence":"additional","affiliation":[{"name":"Department of Pathology Fujita Health University School of Medicine Toyoake"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,6,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v2.i12.852"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4132\/jptm.2015.06.11"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/58.6.642"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/00313020410001692602"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.54.4254"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofx096"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s101470050011"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1075-122X.2004.21319.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1075-122X.2004.21336.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1086\/344463"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/0031302031000082197"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2004.07.013"},{"key":"e_1_2_7_14_1","first-page":"99","article-title":"Idiopathic granulomatous mastitis associated with Corynebacterium sp. infection","volume":"70","author":"Stary CM","year":"2011","journal-title":"Hawaii Med J"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2016.04.023"},{"key":"e_1_2_7_16_1","first-page":"120968","article-title":"Recurrent breast abscesses due to Corynebacterium kroppenstedtii, a human pathogen uncommon in Caucasian women","volume":"2012","author":"Le Fleche\u2010Mateos A","year":"2012","journal-title":"Case Rep Infect Dis"},{"key":"e_1_2_7_17_1","first-page":"4","article-title":"Immunostaining considering the sensitivity instead of the specificity","volume":"34","author":"Tsutsumi Y.","year":"2018","journal-title":"Antibiot Chemother"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03569.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2443975"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64177-6"},{"key":"e_1_2_7_21_1","first-page":"87","article-title":"Molecular diagnosis of medical viruses","volume":"9","author":"Ratcliff RM","year":"2007","journal-title":"Curr Issues Mol Biol"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02160-06"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(00)75126-5"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.03.025"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00760-15"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2016.04.023"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2016.09.006"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1964.tb04528.x"},{"key":"e_1_2_7_29_1","first-page":"7052908","article-title":"Granulomatous lobular mastitis associated with Mycobacterium abscessus in south China: A case report and review of the literature","volume":"2017","author":"Wang Y\u2010S","year":"2017","journal-title":"Case Rep Infect Dis"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(99)00116-5"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.4.1.20"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02121-10"},{"key":"e_1_2_7_33_1","first-page":"296","article-title":"The genus Dietzia: A new home for some known and emerging opportunist pathogens","volume":"55","author":"Koerner RJ","year":"2009","journal-title":"Pathogens Dis"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2012.80"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2014.12.001"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01202-08"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1586\/eri.11.137"}],"container-title":["Pathology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpin.12683","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pin.12683","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T21:27:32Z","timestamp":1695418052000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pin.12683"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,4]]},"references-count":36,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1111\/pin.12683"],"URL":"https:\/\/doi.org\/10.1111\/pin.12683","archive":["Portico"],"relation":{},"ISSN":["1320-5463","1440-1827"],"issn-type":[{"value":"1320-5463","type":"print"},{"value":"1440-1827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,4]]},"assertion":[{"value":"2018-02-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}