{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T20:18:10Z","timestamp":1760300290692,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T00:00:00Z","timestamp":1570060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006120","name":"Ministry of the Environment","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006120","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:p>We previously characterized the morphological characteristics of malignant pleural mesothelioma (MPM) cells with 9p21 homozygous deletion (HD) using a combination of the virtual microscopy and fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH). In this study, we investigated whether MPM cells with BRCA1\u2010associated protein 1 (BAP1) loss show the same morphological characteristics identified in MPM cells with 9p21 HD. MPM cells with either BAP1 loss detected by immunocytochemistry (ICC) or 9p21 HD detected by FISH were identified via virtual microscopy prior to ICC or FISH, followed by analysis and quantification of their morphological characteristics. MPM cells with BAP1 loss or 9p21 HD exhibited significantly more frequent cell\u2010in\u2010cell engulfment, multinucleation, and larger multicellular clusters composed of more than 10 cells than reactive mesothelial cells. In conclusion, MPM cells with BAP1 loss or 9p21 HD share similar cytological features, indicating that the same morphological criteria can be used to detect MPM cells harboring such genetic aberrations.<\/jats:p>","DOI":"10.1111\/pin.12862","type":"journal-article","created":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T09:23:28Z","timestamp":1570094608000},"page":"637-645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Morphological difference between pleural mesothelioma cells in effusion smears with either BAP1 loss or 9p21 homozygous deletion and reactive mesothelial cells without the gene alterations"],"prefix":"10.1111","volume":"69","author":[{"given":"Shinji","family":"Matsumoto","sequence":"first","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and School of Medicine Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Hamasaki","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and School of Medicine Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and School of Medicine Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiaki","family":"Kamei","sequence":"additional","affiliation":[{"name":"Pathology and Cytology Center BML group PCL Japan, Inc. 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