{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:28:52Z","timestamp":1790724532576,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T00:00:00Z","timestamp":1614729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004330","name":"Smoking Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004330","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:p>Breast tissue has a branching structure that contains double\u2010layered cells, consisting primarily of luminal epithelial cells inside and myoepithelial cells outside. Ductal carcinoma <jats:italic>in situ<\/jats:italic> (DCIS) still has myoepithelial cells surrounding the cancer cells. However, myoepithelial cells disappear in invasive ductal carcinoma. In this study, we detected expression of neural EGFL like (NELL) 2 and one of its receptors, roundabout guidance receptor (ROBO) 3, in myoepithelial and luminal epithelial cells (respectively) in normal breast tissue. NELL2 also was expressed in myoepithelial cells surrounding the non\u2010cancerous intraductal proliferative lesions and DCIS. However, the expression level and proportion of NELL2\u2010positive cells in DCIS were lower than those in normal and non\u2010cancerous intraductal proliferative lesions. ROBO3 expression was decreased in invasive ductal carcinoma compared to that in normal and non\u2010cancerous intraductal proliferative lesions. An evaluation of NELL2's function in breast cancer cell lines demonstrated that full\u2010length NELL2 suppressed cell adhesion and migration <jats:italic>in vitro<\/jats:italic>. In contrast, the N\u2010terminal domain of NELL2 increased cell adhesion in the early phase and migration <jats:italic>in vitro<\/jats:italic> in some breast cancer cells. These results suggested that full\u2010length NELL2 protein, when expressed in myoepithelial cells, might serve as an inhibitor of breast cancer cell migration.<\/jats:p>","DOI":"10.1111\/pin.13087","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T21:35:10Z","timestamp":1614807310000},"page":"326-336","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Neural EGFL like 2 expressed in myoepithelial cells and suppressed breast cancer cell migration"],"prefix":"10.1111","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9192-4740","authenticated-orcid":false,"given":"Ritsuko","family":"Nakamura","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Pathology, Graduate School of Medical Sciences Kanazawa University  Ishikawa 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