{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:56:23Z","timestamp":1790751383278,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T00:00:00Z","timestamp":1642550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CBX3 is an isoform of the heterochromatin protein 1 family, which is involved in carcinogenesis and promotes the progression of certain types of cancer. The expression level and clinicopathological significances of CBX3 in colorectal cancer (CRC) are still not well reported. In this study, we examined CBX3 protein expression in formalin\u2010fixed and paraffin\u2010embedded normal mucosae, hyperplastic polyps, low\u2010and high\u2010grade adenomas, and CRC tissue samples using immunohistochemistry. The associations of CBX3 expression levels with clinicopathological parameters, mismatch repair (MMR) protein expression, and kirsten rat sarcoma viral oncogene homolog (KRAS) and B\u2010raf proto\u2010oncogene (BRAF) mutations were analyzed. Our results showed that CBX3 protein was negatively expressed in normal mucosae and hyperplastic polyps, as well as in most low\u2010grade adenomas. Interestingly, CBX3 protein was positively expressed in most high\u2010grade adenomas and CRC tissues. CBX3 expression level was associated with tumor differentiation (<jats:italic>p<\/jats:italic>\u2009=\u20090.012), lymph node metastasis (<jats:italic>p<\/jats:italic>\u2009=\u20090.024), TNM stage (<jats:italic>p<\/jats:italic>\u2009=\u20090.008) and survival (<jats:italic>p<\/jats:italic>\u2009=\u20090.029). CBX3 expression was associated with MMR protein expression (<jats:italic>p<\/jats:italic>\u2009=\u20090.011) and KRAS mutation (<jats:italic>p<\/jats:italic>\u2009=\u20090.013), but not with BRAF mutation (<jats:italic>p<\/jats:italic>\u2009=\u20090.097). Our data suggest that CBX3 may be used as a molecular marker in CRC to evaluate tumor differentiation, lymph node metastasis, and pathological stage.<\/jats:p>","DOI":"10.1111\/pin.13194","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T07:44:09Z","timestamp":1642664649000},"page":"107-116","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Clinicopathological significance of CBX3 in colorectal cancer: An intensive expression study based on formalin\u2010fixed and paraffin\u2010embedded tissues"],"prefix":"10.1111","volume":"72","author":[{"given":"Hai","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Basic Medical Sciences Nanjing Medical University Nanjing China"},{"name":"Center of Pathology and Clinical Laboratory, Sir Run Run Hospital Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8560-3884","authenticated-orcid":false,"given":"Jiandong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology Jinling Clinical Medical College of Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9067-0670","authenticated-orcid":false,"given":"Zhiyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Basic Medical Sciences Nanjing Medical University Nanjing 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