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One hundred and forty\u2010three (95.3%) patients had positive ANA: DNA topoisomerase I (topo I)\u2010like pattern\u201084(56%); speckled pattern\u201044(29.3%);centromere pattern\u20107(4.6%); and nucleolar pattern\u20104(2.6%). Three distinct topo I\u2010like immunofluorescence patterns were detected at 1:40 dilution. Topo I\u2010like pattern (32\/75\u2013limited cutaneous systemic sclerosis (lcSSc) vs. 52\/75\u2010diffuse cutaneous systemic sclerosis (dcSSc); p\u2009&lt;\u20090.001) was found to be associated with dcSSc subset and speckled pattern (lcSSc 28\/75 vs. dcSSc 16\/75; p\u2009&lt;\u20090.03) with lcSSc subset. One hundred and thirty\u2010eight\u00a0(92%) patients were positive for SSc\u2010associated autoAbs. The frequency distribution of autoAbs to topo I, centromere A (CENP A) and centromereB (CENP B), RNA polymerase III (RP11, RP155), fibrillarin (U3RNP), nucleolus organizer region (NOR)\u201090, Th\/To, PM\u2010Scl75, PM\u2010Scl100, Ku, platelet\u2010derived growth factor receptor (PDGFR) and Ro\u201052, were 87(58%), 9(6%), 8(5.3%), 6(4%), 9(6%), 0, 6(4%), 6(4%), 8(5.3%), 5(3.3%), 11(7.3%),0 and 46(30.6%), respectively. Topo I autoAb was strongly associated with dcSSc (35\/75 lcSSc vs. 52\/75 dcSSc; p\u2009&lt;\u20090.004), Raynaud's (p\u2009&lt;\u20090.003), interstitial lung disease (ILD) (p\u2009&lt;\u20090.001) and pulmonary arterial hypertension (PAH) (p\u2009&lt;\u20090.04). This study helps in defining SSc clinical subset, prognostic markers of disease severity, characterization of the topo I\u2010like ANA pattern, and provides a definite association between the ANA patterns and corresponding autoAb.<\/jats:p>","DOI":"10.1111\/pin.13221","type":"journal-article","created":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T13:09:12Z","timestamp":1648040952000},"page":"283-292","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Antinuclear antibody pattern and autoantibody profiling of systemic sclerosis patients in a tertiary referral center in North India"],"prefix":"10.1111","volume":"72","author":[{"given":"Sanghamitra","family":"Machhua","sequence":"first","affiliation":[{"name":"Department of Immunopathology Post Graduate Institute of Medical Education and Research (PGIMER) Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shefali 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