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The diagnosis of sarcoma is challenging because of the lack of specificity of immunohistochemical staining; however, molecular biological approaches, such as genetic mutation, chromosomal translocation, and gene amplification, are promising. In this study, we extracted RNA from formalin\u2010fixed paraffin\u2010embedded (FFPE) tissue derived from surgically resected specimens of sarcoma stored for various periods and performed next\u2010generation sequencing (NGS) analysis by MiniSeq using the Archer Fusion\u2010Plex Sarcoma Panel. RNA was extracted from 63 FFPE tissue samples, and the degree of RNA degradation was assessed. The number of reads and fragment lengths were evaluated by NGS analysis. RNA extraction and cDNA synthesis were successful in 56 cases and library preparation was possible. Fusion genes were detected in 16 of 63 archived FFPE tissue samples in this study. However, in 18 cases, fragmentation was strong, and high\u2010quality libraries could not be obtained. Nevertheless, comprehensive analysis of fusion genes with high sequence specificity by NGS can be a powerful alternative to reverse transcription\u2010polymerase chain reaction and fluorescence in situ hybridization methods.<\/jats:p>","DOI":"10.1111\/pin.13265","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T11:45:36Z","timestamp":1660736736000},"page":"444-456","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of fusion transcripts in sarcoma from archival formalin\u2010fixed paraffin\u2010embedded tissues: A next\u2010generation sequencing approach"],"prefix":"10.1111","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7207-4127","authenticated-orcid":false,"given":"Tomomi","family":"Fujii","sequence":"first","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University School of Medicine Nara 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