{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:33:45Z","timestamp":1790573625186,"version":"4.1.0"},"reference-count":12,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T00:00:00Z","timestamp":1679875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["21ck0106448S0404"],"award-info":[{"award-number":["21ck0106448S0404"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The acquisition of high\u2010quality biospecimens and the appropriate handling of these materials are indispensable for successful clinical sequencing. We developed a cancer clinical sequencing system targeting 160 cancer genes: PleSSision\u2010Rapid. Through the PleSSision\u2010Rapid system, we have analyzed DNA quality evaluated by DIN (DNA integrity number) with 1329 formalin\u2010fixed paraffin embedded (FFPE) samples including 477 prospectively collected tissues for genomic test (P) and 852 archival samples after routine pathological diagnosis (A1\/A2). As a result, the samples with more than DIN 2.1 was 92.0% (439\/477) in prospectively collected sample (P), while it was 85.6% (332\/388) and 76.7% (356\/464) in two types of archival samples (A1\/A2). We performed the PleSSision\u2010Rapid sequence using the samples with over DIN 2.1 and DNA concentration &gt;10\u2009ng\/\u03bcL with which we were able to construct a DNA library, and the probability of sequence success was almost equivalent during all types of specimen processing, at 90.7% (398\/439) in (P), 92.5% (307\/332) in (A1) and 90.2% (321\/356) in (A2), respectively. Our result indicated the clinical benefit to prepare the prospective collection of FFPE materials for indisputable clinical sequence, and that DIN\u2009\u2265\u20092.1 would be a solid parameter for sample preparation of comprehensive genomic profiling tests.<\/jats:p>","DOI":"10.1111\/pin.13318","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T09:28:17Z","timestamp":1679909297000},"page":"198-206","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The DNA integrity number and concentration are useful parameters for successful comprehensive genomic profiling test for cancer using formalin\u2010fixed paraffin embedded tissue"],"prefix":"10.1111","volume":"73","author":[{"given":"Emmy","family":"Yanagita","sequence":"first","affiliation":[{"name":"The Department of Clinical Laboratory Keio University Hospital Tokyo Japan"},{"name":"Clinical Genomic Medicine Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Yamada","sequence":"additional","affiliation":[{"name":"Genomics Unit, Keio Cancer Center Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuro","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"School of Public Health Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eriko","family":"Aimono","sequence":"additional","affiliation":[{"name":"Genomics Unit, Keio Cancer Center Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohei","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Genomics Unit, Keio Cancer Center Keio University School of Medicine Tokyo 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Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/PPO.0000000000000316"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13969"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12631"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atg.2016.06.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqz014"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu376"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.129684.111"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2014.223040"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0176280"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.2144\/btn-2018-0016"}],"container-title":["Pathology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pin.13318","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T12:24:11Z","timestamp":1692447851000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pin.13318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,27]]},"references-count":12,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1111\/pin.13318"],"URL":"https:\/\/doi.org\/10.1111\/pin.13318","archive":["Portico"],"relation":{},"ISSN":["1320-5463","1440-1827"],"issn-type":[{"value":"1320-5463","type":"print"},{"value":"1440-1827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,27]]},"assertion":[{"value":"2022-12-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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