{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:24:28Z","timestamp":1790036668475,"version":"4.0.1"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T00:00:00Z","timestamp":1709856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Papillary renal neoplasm with reverse polarity (PRNRP) is a recently described indolent entity with distinct features and its recognition from other oncocytic\/eosinophilic papillary renal cell carcinoma (ePRCC) has important prognostic implications. ABCC2, a renal drug transporter, is overexpressed in aggressive PRCCs. In this study, we compared the clinicopathological parameters and the biological ABCC2 expression between PRNRP and ePRCC. PRNRP (<jats:italic>n<\/jats:italic>\u2009=\u20098) and ePRCC (<jats:italic>n<\/jats:italic>\u2009=\u200921) cases were selected from resection specimens and corresponding clinicopathological data were collected. ABCC2 immunohistochemical (IHC) staining was performed and ABCC2 staining patterns were classified as negative, cytoplasmic, and brush\u2010border. RNA in\u2010situ hybridization (ISH) was used to assess ABCC2 transcript levels. All eight PRNRP cases had weak cytoplasmic ABCC2 IHC reactivity; however, they showed no detectable ABCC2 transcripts on RNA ISH. In comparison, 76% (16\/21) of ePRCCs showed ABCC2 IHC brush\u2010border expression and significantly higher ABCC2 RNA ISH transcript levels (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Additionally, the ePRCC group showed a significantly larger tumor size (<jats:italic>p<\/jats:italic>\u2009=\u20090.004), higher WHO\/ISUP grade (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), and stage (<jats:italic>p<\/jats:italic>\u2009=\u20090.044). None of the PRNRP cases showed disease progression, while 9.5% (2\/21) ePRCCs had disease progression. PRNRP is clinically and biologically distinct from ePRCC. Hence, it is crucial to differentiate between these two entities, particularly in needle core biopsies.<\/jats:p>","DOI":"10.1111\/pin.13417","type":"journal-article","created":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T13:04:40Z","timestamp":1709903080000},"page":"222-226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Papillary renal neoplasm with reverse polarity is biologically and clinically distinct from eosinophilic papillary renal cell carcinoma"],"prefix":"10.1111","volume":"74","author":[{"given":"Vincent Francis","family":"Castillo","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto Toronto Ontario Canada"},{"name":"Li Ka Shing Knowledge Institute St. Michael's Hospital Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiril","family":"Trpkov","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Alberta Precision Laboratories and University of Calgary Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodorus","family":"Van der Kwast","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto Toronto Ontario Canada"},{"name":"Division of Pathology University Health Network Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Rotondo","sequence":"additional","affiliation":[{"name":"Li Ka Shing Knowledge Institute St. Michael's Hospital Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malek","family":"Hamdani","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Unity Health Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1937-3101","authenticated-orcid":false,"given":"Rola","family":"Saleeb","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto Toronto Ontario Canada"},{"name":"Li Ka Shing Knowledge Institute St. Michael's Hospital Toronto Ontario Canada"},{"name":"Department of Laboratory Medicine Unity Health Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,8]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"World Health Organization classification: urinary and male genital tumours","author":"WHO Classification of Tumours Editorial Board","year":"2022"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001288"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1101268"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-022-01018-6"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1505917"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2021.12.006"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.15042"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2011.08.002"},{"key":"e_1_2_9_10_1","unstructured":"A guide for RNAScope\u00ae Data Analysis [Internet] [cited 2023 Nov 6]. 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