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However, no standardized protocol for CD30 staining has been available. In this study, we compared three common automated immunostaining platforms {Bond III (B III), Dako Omnis (DO) and Ventana BenchMark ULTRA (VBMU)}. A primary antibody for CD30, the Ber\u2010H2 clone, was diluted 50\u2010 to 400\u2010fold for B III and DO, and ready\u2010to\u2010use antibody was used for VBMU. An enhancement step using a linker was introduced in all protocols. First, several candidate dilutions were selected for each platform by staining six cases. These candidate conditions were then confirmed with 60 cases of various types of peripheral T\u2010cell lymphomas (PTCLs). The concordance rates of CD30 expression among platforms differed depending on cutoff values and antibody dilutions, except for anaplastic large cell lymphoma. The concordance rates among three platforms in the evaluation of \u201cpositive\u201d or \u201cnegative\u201d were 100% and 97% when the cutoff values were 1% and 10% respectively, if using 400\u2010diluted antibody in B III and 100\u2010diluted antibody in DO. This study demonstrated the feasibility of equalizing CD30 staining of PTCLs among different platforms by adjusting protocols.<\/jats:p>","DOI":"10.1111\/pin.13472","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T20:18:06Z","timestamp":1724357886000},"page":"530-537","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Standardization of CD30 immunohistochemistry staining among three automated immunostaining platforms"],"prefix":"10.1111","volume":"74","author":[{"given":"Masafumi","family":"Seki","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Graduate School of Medicine Nagoya University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7921-6087","authenticated-orcid":false,"given":"Akira","family":"Satou","sequence":"additional","affiliation":[{"name":"Department of Surgical 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