{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T06:07:08Z","timestamp":1743401228609,"version":"3.37.3"},"reference-count":11,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T00:00:00Z","timestamp":1726444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K14488"],"award-info":[{"award-number":["23K14488"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diagnosing the cause of death can be challenging, particularly for patients with no prior history of visits to the treating hospital. We encountered a case involving a 76\u2010year\u2010old male who was discovered in a state of cardiopulmonary arrest at his home and subsequently declared deceased in our hospital due to severe pneumonia. He had exhibited symptoms of fever over 37\u00b0C and severe coughing for several days. Despite consulting a primary care physician one day prior, his symptoms worsened. Autopsy findings revealed an increase in lung weight and diffuse changes in parenchyma. Histological analysis showed numerous inflammatory cells and exudate within the alveoli. Gram and Periodic acid\u2010Schiff staining were negative, but slight staining was observed in the cytoplasm of macrophages by Warthin\u2010starry and Gimenez stains. Tests using a pan bacterial\/viral detection kit and qualitative polymerase chain reaction (PCR) for <jats:italic>Legionella pneumophila<\/jats:italic> were negative. However, using deoxyribonucleic acid extracted from formalin\u2010fixed paraffin\u2010embedded lung tissue, PCR amplification of the <jats:italic>ssrA<\/jats:italic> gene of congeneric <jats:italic>Legionella<\/jats:italic> species yielded positive results. The results suggest that the cause of death was likely due to bacterial pneumonia caused by <jats:italic>Legionella<\/jats:italic> species.<\/jats:p>","DOI":"10.1111\/pin.13480","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T07:51:11Z","timestamp":1726473071000},"page":"655-659","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of <i>Legionella<\/i> species other than <i>Legionella pneumophila<\/i> in formalin\u2010fixed paraffin\u2010embedded tissue: An autopsy case study"],"prefix":"10.1111","volume":"74","author":[{"given":"Miho","family":"Riku","sequence":"first","affiliation":[{"name":"Department of Pathology Aichi Medical University School of Medicine Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9192-4740","authenticated-orcid":false,"given":"Ritsuko","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Pathology Aichi Medical University School of Medicine Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuguaki","family":"Terashima","sequence":"additional","affiliation":[{"name":"Department of Emergency and Critical Care Medicine Aichi Medical University Hospital Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Sakanashi","sequence":"additional","affiliation":[{"name":"Department of Infection Prevention and Control Aichi Medical University Hospital Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sosuke","family":"Nakata","sequence":"additional","affiliation":[{"name":"Department of Pathology Aichi Medical University School of Medicine Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Department of Pathology Aichi Medical University School of Medicine Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Ohnishi","sequence":"additional","affiliation":[{"name":"Department of Pathology Aichi Medical University School of Medicine Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Pathology Aichi Medical University School of Medicine Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eizo","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Emergency and Critical Care Medicine Aichi Medical University Hospital Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshige","family":"Mikamo","sequence":"additional","affiliation":[{"name":"Department of Infection Prevention and Control Aichi Medical University Hospital Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Kasai","sequence":"additional","affiliation":[{"name":"Department of Pathology Aichi Medical University School of Medicine Nagakute Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,16]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"National Library of Medicine National Center for Biotechnology Information Legionella Taxonomy Browser\u00a0.https:\/\/www.ncbi.nlm.nih.gov\/datasets\/taxonomy\/tree\/?taxon=445. Accessed 30 Apr2024."},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)60078-2"},{"volume-title":"ECDC. Annual Epidemiological Report for 2021","year":"2023","author":"European Centre for Disease Prevention and Control","key":"e_1_2_11_4_1"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.2188\/jea.JE20230178"},{"key":"e_1_2_11_6_1","first-page":"1","article-title":"Legionnaires' disease surveillance, summary report, United State, 2018\u20132019","author":"Barskey A","year":"2022","journal-title":"Divis Bact Dis Nat Center Immunizat Respir Dis"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12911"},{"key":"e_1_2_11_8_1","unstructured":"World Health Organization. Legionellosis.2022.https:\/\/www.who.int\/en\/news-room\/fact-sheets\/detail\/legionellosis. Accessed 30 Apr 2024."},{"key":"e_1_2_11_9_1","unstructured":"MurdochMD StephenTC. Clinical manifestations and diagnosis of Legionella infection Literature review current through. 2022.https:\/\/www.uptodate.com\/contents\/clinical-manifestations-and-diagnosis-of-legionella-infection?search=legionella&source=search_result&selectedTitle=2~119&usage_type=default&display_rank=2"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjresp-2023-001800"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.10.106"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/heart.89.8.839"}],"container-title":["Pathology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pin.13480","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T13:21:30Z","timestamp":1731676890000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pin.13480"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,16]]},"references-count":11,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1111\/pin.13480"],"URL":"https:\/\/doi.org\/10.1111\/pin.13480","archive":["Portico"],"relation":{},"ISSN":["1320-5463","1440-1827"],"issn-type":[{"type":"print","value":"1320-5463"},{"type":"electronic","value":"1440-1827"}],"subject":[],"published":{"date-parts":[[2024,9,16]]},"assertion":[{"value":"2024-05-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}