{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:58:52Z","timestamp":1792191532801,"version":"4.4.0"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The differential diagnosis of epithelioid mesothelioma and reactive mesothelial hyperplasia can be challenging based on morphology alone, particularly in small specimens. Consequently, the use of ancillary techniques, such as immunohistochemistry for BRCA1\u2010associated protein 1, methylthioadenosine phosphorylase, and CDKN2A\/p16 fluorescence in situ hybridization, has been considered a reliable method. Moreover, the potential of Merlin, enhancer of zeste homolog 2, 5\u2010hydroxymethylcytosine, and c\u2010Met immunohistochemistry in differential diagnosis has been reported. We conducted BRCA1\u2010associated protein 1, methylthioadenosine phosphorylase, Merlin, enhancer of zeste homolog 2, 5\u2010hydroxymethylcytosine, and c\u2010Met immunohistochemistry in 43 epithelioid mesothelioma and 28 reactive mesothelial hyperplasia samples. BRCA1\u2010associated protein 1 and\/or methylthioadenosine phosphorylase achieved the highest sensitivity (83.7%), while the addition of Merlin improved the sensitivity to 86.0% while maintaining 100% specificity. We confirmed that the addition of Merlin to BRCA1\u2010associated protein 1 and methylthioadenosine phosphorylase improved the sensitivity in the differential diagnosis of epithelioid mesothelioma and reactive mesothelial hyperplasia while maintaining 100% specificity. Moreover, the utilization of enhancer of zeste homolog 2, 5\u2010hydroxymethylcytosine, and c\u2010Met cannot be unequivocally endorsed, as these markers have not demonstrated 100% specificity, despite their capacity to modestly enhance sensitivity when employed in conjunction with BRCA1\u2010associated protein 1 and methylthioadenosine phosphorylase.<\/jats:p>","DOI":"10.1111\/pin.70069","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T12:26:17Z","timestamp":1766060777000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Merlin Immunohistochemistry Is Useful in the Differential Diagnosis of Epithelioid Mesothelioma and Reactive Mesothelial Hyperplasia"],"prefix":"10.1111","volume":"76","author":[{"given":"Kohei","family":"Aoe","sequence":"first","affiliation":[{"name":"Department of Pathology, Graduate School of Biomedical and Health Sciences Hiroshima University Hiroshima 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