{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T02:48:45Z","timestamp":1789440525729,"version":"build-2803163510"},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>FFPE tissue is essential for molecular testing, but nucleic acid quality is affected by preanalytical factors. Using porcine models, we systematically examined how formalin concentration, fixation duration, cold ischemia time, and tissue size affect DNA and RNA quality. Fresh liver was trimmed to 2, 10, or 50\u2009mm and fixed in 10% or 20% neutral\u2010buffered formalin (NBF) for 1, 3, or 7 days. In small samples, 10% NBF preserved DNA quality better than 20% NBF, while this difference was absent in large samples. RNA quality showed minimal susceptibility to formalin concentration. Both DNA and RNA deteriorated as fixation duration increased from 1 to 3 to 7 days. We next evaluated the impact of post\u2010section storage using clinical FFPE specimens. DNA and RNA fragmentation increased after &gt;\u20091 month and &gt;\u20093 months, respectively, which was prevented by storage at \u2264\u20094\u00b0C. FFPE blocks &lt;\u20091 year old yielded optimal DNA quality. These findings provide practical recommendations: (1) use 10% NBF with \u2264\u200924\u2010h fixation; 20% NBF may be acceptable for large specimens, although its applicability to diverse specimen types remains unverified, (2) select blocks &lt;\u20091 year old, (3) section within 1 month before testing, (4) store sections at \u2264\u20094\u00b0C when storage exceeds 1 month.<\/jats:p>","DOI":"10.1111\/pin.70118","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T05:39:08Z","timestamp":1778564348000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Preanalytical Determinants of DNA and RNA Quality in FFPE Tissues: Practical Recommendations for Molecular Testing"],"prefix":"10.1111","volume":"76","author":[{"given":"Akiko","family":"Kunita","sequence":"first","affiliation":[{"name":"Department of Pathology, Graduate School of Medicine The University of Tokyo Tokyo Japan"},{"name":"Next\u2010Generation Precision Medicine Development Laboratory, Graduate School of Medicine The University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0196-5643","authenticated-orcid":false,"given":"Aya","family":"Shinozaki\u2010Ushiku","sequence":"additional","affiliation":[{"name":"Department of Pathology, Graduate School of Medicine The University of Tokyo Tokyo Japan"},{"name":"Division of Integrative Genomics, Graduate School of Medicine The University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachiyo","family":"Nagumo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Graduate School of Medicine The University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Ushiku","sequence":"additional","affiliation":[{"name":"Department of Pathology, Graduate School of Medicine The University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2013-0691-RA"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2019-0009-SA"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.13170"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.iotech.2021.100069"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqx094"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/jpm12050750"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.13029"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.15790"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.70071"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0203608"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plabm.2024.e00368"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12969"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-025-03307-8"},{"key":"e_1_2_11_15_1","unstructured":"Illumina \u201cEvaluating RNA Quality From FFPE Samples. Guidelines for Obtaining High\u2010Quality RNA Sequencing Results From Degraded RNA With Illumina RNA Enrichment Assays \u201d 2016."}],"container-title":["Pathology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pin.70118","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pin.70118","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pin.70118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:13:49Z","timestamp":1779959629000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pin.70118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":14,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/pin.70118"],"URL":"https:\/\/doi.org\/10.1111\/pin.70118","archive":["Portico"],"relation":{},"ISSN":["1320-5463","1440-1827"],"issn-type":[{"value":"1320-5463","type":"print"},{"value":"1440-1827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2026-02-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70118"}}