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Neoadjuvant subcutaneous administration of combined Pertuzumab and Trastuzumab (Phesgo) was approved in HER2\u2010positive BC. Pathologic complete response (pCR) to neoadjuvant therapy indicates favorable patients' prognosis. Doublecortin\u2010like kinase 1 (DCLK1) could promote chemoresistance. Little is known about the relation of DCLK1 to neoadjuvant therapy response in HER2\u2010positive BC. Assessment of pCR, toxicity profile to Phesgo subcutaneous administration, and DCLK1 immunohistochemical expression in 104 cases of HER2\u2010positive BC were done. Kaplan\u2010Meier curves were used to estimate overall survival (OS) and progression free survival (PFS) in relation to pCR and DCLK1 expression. High DCLK1 expression (46.2%) was associated with large tumor size, grade III tumors, lymph node metastasis, high Ki\u201067 index, and poor neoadjuvant therapy response. pCR (62.5%) was related to older age, negative lymph node status, and low DCLK1 expression. Phesgo was tolerable and didn't cause cardiac toxicity or anaphylaxis. OS and PFS were higher in patients showed pCR and low DCLK1 expression. Dual blockade of HER2 using Phesgo is associated with pCR and better OS\/PFS without adding more toxicity. High DCLK1 expression is related to unfavorable clinicopathologic parameters and poor response to neoadjuvant therapy, together with shorter OS\/PFS.<\/jats:p>","DOI":"10.1111\/pin.70119","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T05:37:23Z","timestamp":1778564243000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Doublecortin\u2010Like Kinase 1 Expression in HER2\u2010Positive Breast Cancer: Relation to Clinicopathological Characteristics and Response to Subcutaneous Injection of Pertuzumab\u2010Trastuzumab (Phesgo) as a Part of Neoadjuvant Chemotherapy"],"prefix":"10.1111","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6184-8078","authenticated-orcid":false,"given":"Radwa Abd El Moneim","family":"Awad","sequence":"first","affiliation":[{"name":"Clinical Oncology and Nuclear Medicine Department, Faculty of Medicine Tanta University Tanta 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