{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:05:21Z","timestamp":1785405921611,"version":"3.56.0"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Subclassification of NSCLC is critical for therapeutic decision\u2010making, particularly in small biopsies and cytology specimens where ancillary testing must be limited. Although the TTF\u20101\/p40 panel is widely used to distinguish adenocarcinoma (ADC) from squamous cell carcinoma, a subset of NSCLCs remains unclassified, highlighting the need for adjunctive markers. Periodic acid\u2010Schiff with diastase (PAS\u2010D) is readily available but underutilized. We retrospectively reviewed 2611 NSCLC specimens from 2298 patients. Diagnosis was based on morphology alone in 38.6% of cases, while ancillary testing was required in 57.7%. Among 287 TTF\u20101\n                    <jats:sub>neg<\/jats:sub>\n                    \/p40\n                    <jats:sub>neg<\/jats:sub>\n                    tumors, 30.3% were classified as ADC using PAS\u2010D and\/or Napsin A. PAS\u2010D demonstrated higher sensitivity than Napsin A (71.8% vs. 69.3%), and markedly outperformed Napsin A in TTF\u20101\u2010negative ADCs (89.7% vs. 25.9%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). This finding reflects biological differences between terminal respiratory unit\u2010derived ADCs and non\u2010terminal airway tumors, in which PAS\u2010D positivity is more diagnostically informative. Combined testing improved sensitivity, with the TTF\u20101\/PAS\u2010D panel achieving 97.5%. This study highlights the underappreciated diagnostic value of PAS\u2010D, particularly in TTF\u20101\u2010negative tumors. PAS\u2010D represents a simple, effective adjunct to routine diagnostic panels. Therefore,\u00a0incorporating PAS\u2010D into routine panels can enhance NSCLC subclassification, particularly in TTF\u20101\n                    <jats:sub>neg<\/jats:sub>\n                    \/p40\n                    <jats:sub>neg<\/jats:sub>\n                    tumors and support accurate diagnosis and optimal clinical management.\n                  <\/jats:p>","DOI":"10.1111\/pin.70154","type":"journal-article","created":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T09:16:52Z","timestamp":1785057412000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Re\u2010appraisal of the Diagnostic Value of PAS\u2010D Staining in TTF\u20101\u2010NEGATIVE NSCLC Subclassification: A Large Real\u2010World Cohort Study"],"prefix":"10.1111","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7671-0100","authenticated-orcid":false,"given":"Dogu","family":"Vuralli Bakkaloglu","sequence":"first","affiliation":[{"name":"Department of Pathology, Istanbul Faculty of Medicine Istanbul University Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2625-0070","authenticated-orcid":false,"given":"Hafize","family":"Ozdemir","sequence":"additional","affiliation":[{"name":"Department of Pathology, Istanbul Faculty of Medicine Istanbul University Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3425-2137","authenticated-orcid":false,"given":"Melek","family":"Buyuk","sequence":"additional","affiliation":[{"name":"Department of Pathology, Istanbul Faculty of Medicine Istanbul University Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilek","family":"Yilmazbayhan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Istanbul Faculty of Medicine Istanbul University Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasemin","family":"Ozluk","sequence":"additional","affiliation":[{"name":"Department of Pathology, Istanbul Faculty of Medicine Istanbul University Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/jco.2008.26.15_suppl.7508"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2004.11.022"},{"key":"e_1_2_11_4_1","volume-title":"WHO Classification Thoracic Tumours","author":"WHO 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