{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:03:13Z","timestamp":1790694193362,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Peritoneal lavage cytology (PLC) is important for staging gastric cancer, but intraoperative assessment may miss malignant cells because only a limited portion of the specimen can be examined. We retrospectively analyzed 287 patients who underwent gastrectomy with intraoperative PLC between 2014 and 2022. Cytologic status was classified as CY\u2212, CY0\u2010to\u2010CY1 conversion, or CY+ based on intraoperative and permanent cytologic findings. Of 287 patients, 251 were CY\u2212, 10 showed CY0\u2010to\u2010CY1 conversion, and 26 were CY+ on intraoperative assessment, resulting in 36 final CY1 cases. Patients with CY0\u2010to\u2010CY1 conversion had significantly worse overall survival than CY\u2212 patients and outcomes comparable to those of CY+ patients. Serosal invasion\/pT4 disease was strongly associated with final CY1 status and was frequent among conversion cases. In a separate technical evaluation, brief ethanol fixation followed by polyethylene glycol and acetone\u2013methanol fixation (Et\u2009+\u2009PA fixation) significantly improved total cell retention and preservation of tumor\u2010cell clusters compared with conventional ethanol fixation in 11 paired cases. CY0\u2010to\u2010CY1 conversion represents clinically meaningful cytology\u2010positive disease. Et\u2009+\u2009PA fixation improves cellular retention during cytologic preparation and may improve the quality of intraoperative PLC preparations.<\/jats:p>","DOI":"10.1111\/pin.70173","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T06:29:41Z","timestamp":1789021781000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CY0\u2010to\u2010CY1 Conversion in Gastric Cancer Peritoneal Lavage Cytology: Clinical Significance and Improved Cell Retention by Et\u2009+\u2009PA Fixation"],"prefix":"10.1111","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0712-7542","authenticated-orcid":false,"given":"Akihiko","family":"Fukagawa","sequence":"first","affiliation":[{"name":"Department of Pathology, Graduate School of Medicine The University of Tokyo Tokyo Japan"},{"name":"Division of Systems Epidemiology National Cancer Center Research Institute Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nao","family":"Yoshizawa","sequence":"additional","affiliation":[{"name":"Department of Surgery Showa General Hospital Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4914-4544","authenticated-orcid":false,"given":"Shun","family":"Iwai","sequence":"additional","affiliation":[{"name":"Junior Residency Program Showa General Hospital Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2509-8641","authenticated-orcid":false,"given":"Kosei","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Junior Residency Program Showa General Hospital Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kota","family":"Itamoto","sequence":"additional","affiliation":[{"name":"Department of Surgery Showa General Hospital Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Wakabayashi","sequence":"additional","affiliation":[{"name":"Department of Pathology Showa General Hospital Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Hamakawa","sequence":"additional","affiliation":[{"name":"Department of Pathology Showa General Hospital Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Surgery Showa General Hospital Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taichiro","family":"Yoshimoto","sequence":"additional","affiliation":[{"name":"Department of Pathology Showa General Hospital Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-017-0749-y"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v9.i32.9711"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-006-0406-3"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-005-0343-6"},{"key":"e_1_2_10_7_1","unstructured":"Japanese Gastric Cancer Association. Japanese Gastric Cancer Treatment Guidelines(Japanese Gastric Cancer Association;2025)."},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-40618-3"},{"key":"e_1_2_10_9_1","volume-title":"Japanese Classification of Gastric Carcinoma","author":"Japanese Gastric Cancer Association","year":"2026"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9098(199910)72:2<60::AID-JSO3>3.0.CO;2-1"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.7812"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2388(199909)17:2<103::AID-SSU4>3.0.CO;2-Q"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.25259\/CMAS_03_18_2021"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12156"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66637-y"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.5230\/jgc.2013.13.4.214"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/AS9.0000000000000607"}],"container-title":["Pathology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pin.70173","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pin.70173","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pin.70173","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:51:52Z","timestamp":1790693512000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pin.70173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":16,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/pin.70173"],"URL":"https:\/\/doi.org\/10.1111\/pin.70173","archive":["Portico"],"relation":{},"ISSN":["1320-5463","1440-1827"],"issn-type":[{"value":"1320-5463","type":"print"},{"value":"1440-1827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70173"}}